Abstract：
The present study was conducted with the aim of isolation and identification of the biofilm-forming denitrifying Pseudomonas bacterial strains from eutrophic waters of Dal lake, India, followed by the study of inter-relation of biofilm formation and denitrification potential of Pseudomonas strains. The bacterial strains were characterized by morphological observations and identified using 16S rDNA sequencing followed by the quantification of biofilm formation of these st by crystal violet (CV) assay using 96-well microtiter plate and extracellular polymeric substance (EPS) extraction. Lastly, the nitrate-reducing potential of all Pseudomonas species was studied. Our evaluation revealed that four different Pseudomonas species were observed to have the biofilm-forming potential and nitrate-reducing properties and the species which showed maximum biofilm-forming potential and maximum EPS production exhibited higher nitrate-removing capacity. Moreover, P. otitis was observed to have the highest denitrification capacity (89%) > P. cedrina (83%) > P. azotoform (79%) and the lowest for P. peli (70%). These results clearly signify a positive correlation of biofilm-forming capacity and nitrate-removing ability of Pseudomonas species. This study has for the first time successfully revealed the bioremediation potential of P. otitis, P. cedrina, P. azotoform, and P. peli species, thus contributing to the growing list of known nitrate-reducing Pseudomonas species. Based upon the results, these strains can be extrapolated to nitrate-polluted water systems for combating water pollution.
摘要：
本研究旨在从达尔湖的富营养化水域中分离和鉴定形成生物膜的反硝化假单胞菌菌株，印度,其次研究了假单胞菌菌株生物膜形成与反硝化潜力的相互关系。通过形态学观察对细菌菌株进行表征，并使用16SrDNA测序进行鉴定，然后通过使用96孔微量滴定板和细胞外聚合物（EPS）提取的结晶紫（CV）测定法对这些st的生物膜形成进行定量。最后,研究了所有假单胞菌物种的硝酸盐还原潜力。我们的评估表明，观察到四种不同的假单胞菌物种具有生物膜形成潜力和硝酸盐还原特性，并且显示出最大生物膜形成潜力和最大EPS产量的物种显示出较高的硝酸盐去除能力。此外，观察到P.中耳炎具有最高的反硝化能力(89%)>P.cedrina(83%)>P.偶氮型(79%)和最低的P.peli(70%)。这些结果清楚地表明假单胞菌物种的生物膜形成能力和硝酸盐去除能力呈正相关。这项研究首次成功揭示了耳炎的生物修复潜力，P.Cedrina,P.偶氮型,和P.peli物种，从而导致已知的硝酸盐还原假单胞菌物种的不断增加。根据结果,这些菌株可以外推到硝酸盐污染的水系统中，以对抗水污染。
