PDF(Pubmed)
Abstract：
Controlling the activity of DNAzymes by external triggers is an important task. Here a temporal control over DNAzyme activity through a mechanochemical pathway with the help of ultrasound (US) is demonstrated. The deactivation of the DNAzyme is achieved by hybridization to a complementary strand generated through rolling circle amplification (RCA), an enzymatic polymerization process. Due to the high molar mass of the resulting polynucleic acids, shear force can be applied on the RCA strand through inertial cavitation induced by US. This exerts mechanical force and leads to the cleavage of the base pairing between RCA strand and DNAzyme, resulting in the recovery of DNAzyme activity. This is the first time that this release mechanism is applied for the activation of catalytic nucleic acids, and it has multiple advantages over other stimuli. US has higher penetration depth into tissues compared to light, and it offers a more specific stimulus than heat, which has also limited use in biological systems due to cell damage caused by hyperthermia. This approach is envisioned to improve the control over DNAzyme activity for the development of reliable and specific sensing applications.
摘要：
通过外部触发物控制DNAzyme的活性是一项重要任务。这里证明了在超声（US）的帮助下通过机械化学途径对DNAzyme活性的时间控制。DNA酶的失活是通过与通过滚环扩增(RCA)产生的互补链杂交来实现的。酶聚合过程。由于所得多核酸的高摩尔质量，剪切力可以通过US引起的惯性空化作用在RCA钢绞线上。这会施加机械力并导致RCA链和DNA酶之间的碱基配对的裂解，导致DNA酶活性的恢复。这是首次将这种释放机制应用于催化核酸的活化，它比其他刺激有多重优势。与光相比，美国对组织的穿透深度更高，它提供了比热更具体的刺激，由于热疗引起的细胞损伤，其在生物系统中的使用也受到限制。设想这种方法改善对DNA酶活性的控制,以开发可靠和特定的传感应用。
