Abstract：
This work aimed to investigate the microbial mechanisms for the improvement of composting efficiency driven by the compound microbial inoculum (MI) (Bacillus subtilis SL-44, Enterobacter hormaechei Rs-189 and Trichoderma reesei) during co-composting of spent mushroom substrate (SMS) and chicken manure (CM). The treatments used in the study were as follows: 1) MI (inoculation with microbial inoculum), 2) CI (inoculation with commercial microbial inoculum), and 3) CK (without inoculation). The results demonstrated that MI increased the seed germination index (GI) by 25.11%, and contents of humus, humic acid (HA) and available phosphorus (AP) were correspondingly promoted by 12.47%, 25.93% and 37.16%, respectively. The inoculation of MI increased the temperature of the thermophilic stage by 3-7 °C and achieved a cellulose degradation rate of 52.87%. 16S rRNA gene analysis indicated that Actinobacteria (11.73-61.61%), Firmicutes (9.46-65.07%), Proteobacteria (2.86-32.17%) and Chloroflexi (0.51-10.92%) were the four major phyla during the inoculation composting. Bacterial metabolic functional analysis revealed that pathways involved in amino acid and glycan biosynthesis and metabolism were boosted in the thermophilic phase. There was a positive correlation between bacterial communities and temperature, humification and phosphorus fractions. The average dry weight, fresh weight and seedling root length in the seedling substrates adding MI compost were 1.13, 1.23 and 1.06 times higher than those of the CK, respectively. This study revealed that biological inoculation could improve the composting quality and efficiency, potentially benefiting the resource utilization of agricultural waste resources.
摘要：
这项工作旨在研究复合微生物接种物（MI）（枯草芽孢杆菌SL-44，ormaechei肠杆菌Rs-189和里氏木霉）在废蘑菇基质（SMS）和鸡粪（CM）共同堆肥过程中提高堆肥效率的微生物机理。研究中使用的处理方法如下:1)MI(接种微生物接种物)，2)CI(用商业微生物接种物接种)，和3)CK(未接种)。结果表明，MI使种子发芽指数（GI）提高了25.11％，和腐殖质的内容，腐殖酸（HA）和有效磷（AP）相应地提高了12.47％，25.93%和37.16%，分别。MI的接种使嗜热阶段的温度提高了3-7°C，纤维素降解率为52.87％。16SrRNA基因分析表明放线菌(11.73-61.61%),Firmicutes(9.46-65.07%),在接种堆肥过程中，变形菌（2.86-32.17％）和氯氟菌（0.51-10.92％）是四个主要的门。细菌代谢功能分析表明，在嗜热阶段，参与氨基酸和聚糖生物合成和代谢的途径得到加强。细菌群落与温度呈正相关,腐殖质和磷馏分。平均干重，添加MI堆肥的幼苗基质中的鲜重和幼苗根长分别比CK高1.13、1.23和1.06倍，分别。本研究表明，生物接种可以提高堆肥质量和效率，可能有利于农业废弃物资源的资源化利用。
