Abstract：
Particulate matter (PM) exposure may be associated with male semen quality. Besides, PM exposure induces up and down levels of trace metals in tissues or organs. The levels of trace metals in semen are critical for adverse male semen quality. This study aims to evaluate the concentrations of seminal-level trace metals in fertile men and assess its associations with PM exposure and to explore the mediation role of trace metals in seminal plasma plays in the relationship between PM exposure and semen quality. Total 1225 fertile men who participated in a cohort study from 2014 to 2016 were finally recruited. Multivariate linear regression was applied to explore associations between each two of PM exposure, trace metals and semen parameters. 1-year PM2.5 and PM10 exposure levels were positively associated with arsenic (As), mercury (Hg), lanthanum (La), praseodymium (Pr), neodymium (Nd) but negatively associated with vanadium (V), magnesium (Mg), strontium (Sr), barium (Ba) in semen. It was also found that most of the elements were associated with total sperm number, followed by sperm concentration. Redundancy analysis (RDA) also determined several strong positive correlations or negative correlations between 1-year PM exposure and trace metals. Mediation analysis found that trace metals had a potentially compensatory or synergetic indirect effect on the total effect of the association between 1-year PM exposure and semen quality. The retrospective cohort study provides long-term PM exposure that may cause abnormal semen quality by affecting seminal plasma element levels.
摘要：
颗粒物（PM）暴露可能与男性精液质量有关。此外,PM暴露会导致组织或器官中痕量金属的水平升高和降低。精液中微量金属的含量对男性精液质量不利至关重要。本研究旨在评估可育男性精液水平痕量金属的浓度，并评估其与PM暴露的关系，并探讨精浆中痕量金属在PM暴露与精液质量之间的关系中的中介作用。最终招募了2014年至2016年参加队列研究的1225名育龄男性。采用多元线性回归方法探索每两个PM暴露之间的关联，痕量金属和精液参数。1年PM2.5和PM10暴露水平与砷（As）呈正相关，汞(Hg),镧(La),镨(Pr),钕（Nd）但与钒（V）呈负相关，镁(Mg),锶(Sr),精液中的钡（Ba）。还发现大多数元素与精子总数有关，其次是精子浓度。冗余分析（RDA）还确定了1年PM暴露与痕量金属之间的几种强正相关或负相关。中介分析发现，痕量金属对1年PM暴露与精液质量之间关联的总效应具有潜在的补偿或协同间接效应。回顾性队列研究提供了长期PM暴露，可能通过影响精浆元素水平而导致精液质量异常。
