Abstract：
The complete genome sequence of a putative novel potyvirus, tentatively named \"polygonatum kingianum mottle virus\" (PKgMV; GenBank accession no. ON428226), infecting Polygonatum kingianum in China, was obtained by next-generation sequencing (NGS), reverse transcription polymerase chain reaction (RT-PCR), and rapid amplification of cDNA ends (RACE). PKgMV exhibits the typical genome organization and characteristics of members of the genus Potyvirus, with a length of 10,002 nucleotides (nt) and a large open reading frame (nt 108 to 9,746) encoding a polyprotein of 3,212 amino acids (aa) (363.68 kDa). Pairwise comparisons revealed that the PKgMV polyprotein shares 50.5-68.6% nt and 43.1-72.2% aa sequence identity with reported members of the genus Potyvirus. Moreover, phylogenetic analysis indicated that PKgMV is closely related to polygonatum kingianum virus 1 (PKgV1; accession no. MK427056). These results suggest that the PKgMV is a novel member of the genus Potyvirus of the family Potyviridae.
摘要：
一种推定的新型马铃薯病毒的完整基因组序列，暂定名为“黄精斑驳病毒”(PKgMV；GenBank登录号ON428226),在中国感染黄精，通过下一代测序(NGS)获得，逆转录聚合酶链反应(RT-PCR),和cDNA末端的快速扩增(RACE)。PKgMV表现出典型的基因组组织和Potyvirus属成员的特征，长度为10,002个核苷酸（nt）和一个大的开放阅读框（nt108至9,746），编码3,212个氨基酸（aa）（363.68kDa）的多蛋白。成对比较显示，PKgMV多蛋白与报告的Potyvirus属成员具有50.5-68.6％nt和43.1-72.2％aa序列同一性。此外，系统发育分析表明，PKgMV与黄精病毒1密切相关（PKgV1；登录号MK427056)。这些结果表明PKgMV是Potyviridae家族Potyvirus属的新成员。
