Abstract：
Withanolides are the class of steroidal molecules getting greater emphasis in recent years. Quality control throughout the manufacturing and storage period is often one of the key problems that have restricted their broad use in India\'s indigenous and Ayurvedic medical systems for thousands of years. Because of their diverse clinical potential, withanolides have received a great deal of scientific attention. Analytical techniques are being devised for the automated isolation, identification, and estimation of every single protein within the cell as well as in herbal extracts of withanolides, due to which now researchers are interested in determining the effects of metabolism as well as various stimuli on protein expression, which made the study easier. This study discusses the potential use of hyphenated analytical methods that are reliable in understanding the molecular signaling features, proteome evaluation and characterization of withanolides, in addition to examining existing methodological limitations. The choice of analytical techniques for the withanolides analysis, however, relies on the nature of the sample matrix, the aim of the analysis, and the sensitivity of the technique.
摘要：
Withanolides是近年来越来越受到重视的一类甾体分子。在整个制造和储存期间的质量控制通常是限制其在印度本土和阿育吠陀医疗系统中广泛使用数千年的关键问题之一。由于它们不同的临床潜力，Nivolides已经受到了大量的科学关注。分析技术正在设计用于自动分离，identification,估计细胞内的每一种蛋白质以及草药提取物中的各种蛋白质，因此，现在研究人员对确定新陈代谢以及各种刺激对蛋白质表达的影响感兴趣，这使得研究更容易。本研究讨论了在理解分子信号特征方面可靠的连字符分析方法的潜在用途。Nutanolides的蛋白质组评估和表征，除了检查现有的方法限制。选择的分析技术，然而,依赖于样本矩阵的性质，分析的目的，和技术的灵敏度。
