PDF(Pubmed)
Abstract：
Nasal obstruction exerts considerable physiological effects on the respiratory system and craniofacial morphology during the developmental stage. This study used MMP-3-LUC transgenic rats for in vivo tracking of long-term expression in the rat nasal region after unilateral nasal obstruction. Skeletal changes of the craniofacial, nasal, and sinus regions were measured through micro-computed tomography examination and analysis with 3D image processing and calculation. Matrix metalloproteinase-3 and olfactory marker protein expression were also investigated through immunohistochemistry (IHC). Unilateral nasal obstruction significantly reduced the MMP-3 signal in the nasal region of MMP-3-LUC transgenic rats, which was mainly expressed in the respiratory epithelium. Long-term obstruction also caused morphological changes of the craniofacial hard tissue, such as nasal septal deviation, longer inter-jaw distance, and increased maxillary molar dental height. It also caused compensatory growth in olfactory nerve bundles and the olfactory epithelium, as confirmed by IHC. In our study, long-term unilateral nasal obstruction caused nasal septal deviation toward the unobstructed side, hyper divergent facial development including longer molar dental height, and reduced MMP-3 production. However, further investigation is necessary to explore the mechanism in depth.
摘要：
鼻塞在发育阶段对呼吸系统和颅面形态产生相当大的生理影响。本研究使用MMP-3-LUC转基因大鼠进行单侧鼻塞后大鼠鼻区长期表达的体内追踪。颅面的骨骼变化，鼻部,通过显微计算机断层扫描检查和3D图像处理和计算分析来测量和鼻窦区域。还通过免疫组织化学（IHC）研究了基质金属蛋白酶3和嗅觉标记蛋白的表达。单侧鼻塞显著降低MMP-3-LUC转基因大鼠鼻区MMP-3信号,主要在呼吸道上皮中表达。长期梗阻还引起颅面硬组织的形态学改变，比如鼻中隔偏曲,颌间距离较长，上颌磨牙的牙齿高度增加。它还引起嗅觉神经束和嗅觉上皮的代偿性生长，IHC证实。在我们的研究中,长期单侧鼻塞导致鼻中隔偏向通畅侧，超发散的面部发育，包括更长的磨牙牙齿高度，减少MMP-3的产生。然而,有必要进一步调查，深入探索机制。
