Abstract：
Neutrophils play a pivotal role in innate immunity by releasing ROS through respiratory bursts to neutralize various pathogenic factors. However, excessive ROS release can cause tissue damage. Adenosine is an endogenous anti-inflammatory molecule inhibiting respiratory burst to protect the host. Adenosine aptamers with antibody-like properties and good stability are expected to act as adenosine antagonists with functional modulation capability. This study compares the effects of adenosine and its aptamer on the respiratory bursts of salivary polymorphonuclear leukocytes and circulating polymorphonuclear leukocytes using a programmable stopped-flow injection approach, ensuring rapid and efficient analysis while maintaining the neutrophils\' viability. The results show that primed salivary polymorphonuclear leukocytes exhibit specificities that differ from circulating polymorphonuclear leukocytes. Adenosine aptamer can function as an inhibitory antagonist that distinguishes between physiologically controlled and excessive priming of neutrophils, showing potential application prospects in immunotherapy.
摘要：
中性粒细胞通过呼吸爆发释放ROS来中和各种致病因子，在先天免疫中起关键作用。然而,过量的ROS释放会导致组织损伤。腺苷是抑制呼吸爆发以保护宿主的内源性抗炎分子。预期具有抗体样性质和良好稳定性的腺苷适体充当具有功能调节能力的腺苷拮抗剂。这项研究比较了腺苷及其适体对唾液多形核白细胞和循环多形核白细胞呼吸爆发的影响，使用可编程的停流注射方法，确保快速有效的分析，同时保持中性粒细胞的活力。结果表明，引发的唾液多形核白细胞表现出与循环多形核白细胞不同的特异性。腺苷适体可以作为抑制性拮抗剂，区分生理控制和过度引发中性粒细胞，在免疫治疗方面显示出潜在的应用前景。
