Abstract：
Antibiotic administration is an adjacent therapy to guided tissue regeneration (GTR) in the management of periodontitis. This is due to the major role of pathogen biofilm in aggravating periodontal defects. This study aimed to fabricate a GTR membrane for sustained delivery of doxycycline hydrochloride (DOX) while having a space-maintaining function. The membranes were prepared using a polymeric blend of polycaprolactone/polyvinyl alcohol/chitosan by the electrospinning technique. The obtained membranes were characterized in terms of physicochemical and biological properties. Nanofibers showed a mean diameter in the submicron range of < 450 nm while having uniform randomly aligned morphology. The obtained membranes showed high strength and flexibility. A prolonged in vitro release profile during 68 h was observed for manufactured formulations. The prepared membranes showed a cell viability of > 70% at different DOX concentrations. The formulations possessed antimicrobial efficacy against common pathogens responsible for periodontitis. In vivo evaluation also showed prolonged release of DOX for 14 days. The histopathological evaluation confirmed the biocompatibility of the GTR membrane. In conclusion, the developed nanofibrous DOX-loaded GTR membranes may have beneficial characteristics in favour of both sustained antibiotic delivery and periodontal regeneration by space-maintaining function without causing any irritation and tissue damage.
摘要：
抗生素给药是牙周炎治疗中指导组织再生（GTR）的相邻疗法。这是由于病原体生物膜在加重牙周缺损中的主要作用。本研究旨在制造用于持续递送盐酸多西环素(DOX)同时具有空间保持功能的GTR膜。通过静电纺丝技术使用聚己内酯/聚乙烯醇/壳聚糖的聚合物共混物制备膜。所获得的膜在物理化学和生物学性质方面进行了表征。纳米纤维显示出<450nm的亚微米范围内的平均直径,同时具有均匀的随机排列的形态。所获得的膜显示出高强度和柔性。对于制造的制剂，观察到在68小时内延长的体外释放曲线。制备的膜在不同DOX浓度下显示>70%的细胞活力。该制剂对引起牙周炎的常见病原体具有抗微生物功效。体内评估也显示了14天的DOX的延长释放。组织病理学评价证实了GTR膜的生物相容性。总之,开发的负载DOX的纳米纤维GTR膜可能具有有利于持续抗生素递送和通过空间保持功能的牙周再生而不引起任何刺激和组织损伤的有益特征。
