PDF(Pubmed)
Abstract：
UNASSIGNED: Diversity in clinical signs and symptoms are associated with soil transmitted diseases (STD), which are spread to humans by intestinal worms and transmitted in a variety of ways. There is a need for the present study, which aimed to investigate the prevalence of intestinal parasites and to compare between the common detection techniques for soil-transmitted helminths (STHs) among newly arrived expatriate labors in Jeddah, Saudi Arabia.
UNASSIGNED: A total of 188 stool samples were analyzed by macroscopic examination, and microscopic examination using direct iodine smear and the formal ether sedimentation technique. Trichrome and modified Kinyoun\'s stains were used to confirm the morphology of any detected protozoa stages and oocyst of Cryptosporidium, respectively. A chromatographic immunoassay kit was used for Entamoeba histolytica, Giardia lamblia and Cryptosporidium. In addition, real-time PCR was employed only to identify various STHs.
UNASSIGNED: Out of 188, several types of parasites were detected in 35 samples (18.62%), of which some with multiple infections. Nine samples (4.79%) were positive for Entamoeba coli, seven samples (3.72%) for Trichuris trichiura, six samples (3.19%) for Necator americanus, four samples (2.13%) for Strongyloides stercoralis, four samples (2.13%) for Ascaris lumbricoides, four samples (2.13%) for E. histolytica, three samples (1.60%) for Blastocystis hominis and two samples (1.06%) for Ancylostoma duodenale. In comparison between laboratory techniques for STHs, real-time PCR was able to detect the DNA of 19 samples (10.1%) followed by Ritchie sedimentation technique (18, 9.6%), and direct smear (7, 3.7%) (p > 0.05).
UNASSIGNED: The high rate of newly arrived foreign workers infected with intestinal parasites could lead to a risk to society. Continuous and regular surveys are needed to deal with the occurrence of intestinal parasitic infections including STHs. To improve the identification of these infections, we recommend a supporting infrastructure for the application of concentration methods and molecular assays.
UNASSIGNED: A total of 188 stool samples were analyzed by macroscopic examination, and microscopic examination using direct iodine smear and the formal ether sedimentation technique. Trichrome and modified Kinyoun\'s stains were used to confirm the morphology of any detected protozoa stages and oocyst of Cryptosporidium, respectively. A chromatographic immunoassay kit was used for Entamoeba histolytica, Giardia lamblia and Cryptosporidium. In addition, real-time PCR was employed only to identify various STHs.
UNASSIGNED: Out of 188, several types of parasites were detected in 35 samples (18.62%), of which some with multiple infections. Nine samples (4.79%) were positive for Entamoeba coli, seven samples (3.72%) for Trichuris trichiura, six samples (3.19%) for Necator americanus, four samples (2.13%) for Strongyloides stercoralis, four samples (2.13%) for Ascaris lumbricoides, four samples (2.13%) for E. histolytica, three samples (1.60%) for Blastocystis hominis and two samples (1.06%) for Ancylostoma duodenale. In comparison between laboratory techniques for STHs, real-time PCR was able to detect the DNA of 19 samples (10.1%) followed by Ritchie sedimentation technique (18, 9.6%), and direct smear (7, 3.7%) (p > 0.05).
UNASSIGNED: The high rate of newly arrived foreign workers infected with intestinal parasites could lead to a risk to society. Continuous and regular surveys are needed to deal with the occurrence of intestinal parasitic infections including STHs. To improve the identification of these infections, we recommend a supporting infrastructure for the application of concentration methods and molecular assays.
摘要：
■临床体征和症状的多样性与土壤传播疾病（STD）有关，它们通过肠道蠕虫传播给人类，并以多种方式传播。有必要进行本研究，旨在调查肠道寄生虫的患病率，并比较吉达新来的外籍劳工中土壤传播蠕虫（STHS）的常用检测技术，沙特阿拉伯。
■通过宏观检查分析了总共188个粪便样本，使用直接碘涂片和正规乙醚沉降技术进行显微镜检查。三色染色和改良的Kinyoun染色用于确认任何检测到的隐孢子虫原生动物阶段和卵囊的形态，分别。色谱免疫分析试剂盒用于溶组织内阿米巴，蓝氏贾第鞭毛虫和隐孢子虫。此外,实时PCR仅用于鉴定各种STH。
■在188个样本中，在35个样本（18.62％）中检测到几种类型的寄生虫，其中一些患有多种感染。9份样本(4.79%)为大肠杆菌内阿米巴阳性,七个样本(3.72%)为Trichiura，美洲Necator的六个样本（3.19％），4个样本（2.13%）为类圆线虫，四个样本(2.13%)为蛔虫，四个样本（2.13%）为溶组织大肠杆菌，人芽囊原虫3个样本（1.60％），十二指肠囊造孔2个样本（1.06％）。在比较实验室技术之间的STHS，实时PCR能够检测到19个样本的DNA（10.1％），然后采用Ritchie沉降技术（18，9.6％），和直接涂片(7，3.7%)(p>0.05)。
■新到达的外国工人感染肠道寄生虫的高比率可能会给社会带来风险。需要进行连续和定期的调查以应对包括STH在内的肠道寄生虫感染的发生。为了提高对这些感染的识别，我们建议为浓缩方法和分子分析的应用提供支持的基础设施。
■通过宏观检查分析了总共188个粪便样本，使用直接碘涂片和正规乙醚沉降技术进行显微镜检查。三色染色和改良的Kinyoun染色用于确认任何检测到的隐孢子虫原生动物阶段和卵囊的形态，分别。色谱免疫分析试剂盒用于溶组织内阿米巴，蓝氏贾第鞭毛虫和隐孢子虫。此外,实时PCR仅用于鉴定各种STH。
■在188个样本中，在35个样本（18.62％）中检测到几种类型的寄生虫，其中一些患有多种感染。9份样本(4.79%)为大肠杆菌内阿米巴阳性,七个样本(3.72%)为Trichiura，美洲Necator的六个样本（3.19％），4个样本（2.13%）为类圆线虫，四个样本(2.13%)为蛔虫，四个样本（2.13%）为溶组织大肠杆菌，人芽囊原虫3个样本（1.60％），十二指肠囊造孔2个样本（1.06％）。在比较实验室技术之间的STHS，实时PCR能够检测到19个样本的DNA（10.1％），然后采用Ritchie沉降技术（18，9.6％），和直接涂片(7，3.7%)(p>0.05)。
■新到达的外国工人感染肠道寄生虫的高比率可能会给社会带来风险。需要进行连续和定期的调查以应对包括STH在内的肠道寄生虫感染的发生。为了提高对这些感染的识别，我们建议为浓缩方法和分子分析的应用提供支持的基础设施。
