Abstract：
Bacterial infection has been regarded as a life-threatening problem in clinic. In addition to screening of new antibiotics, it is important to develop highly effective antibacterial materials against antibiotic resistance with capacities on modulating chronic inflammation. Herein, aligned Chlorin e6 (Ce6) conjugated silk fibroin electrospun fibers were successfully fabricated on silk fibroin based film via electrospining to achieve effective photodynamic antibacterial activities under near infrared (NIR) irradiation. The aligned electrospun fiber based film composite (SFCF@Film) exhibited good mechanical properties and desirable hemocompatibility. SFCF@Film provided a promising guidance cue for directing cell orientation and promoting cell growth. Significantly, SFCF@Film effectively generated ROS under NIR irradiation to kill S. aureus for treating wound infections within 10 min and promoted M2 polarization of macrophages for wound healing at later stage. Therefore, we believed that this engineered bioscaffold can be a powerful strategy for handling wound infection.
摘要：
细菌感染已被认为是临床上威胁生命的问题。除了筛选新的抗生素，开发具有调节慢性炎症能力的抗抗生素耐药性的高效抗菌材料是重要的。在这里，在基于丝素蛋白的薄膜上，通过静电纺丝成功地制备了取向的氯e6（Ce6）共轭丝素电纺纤维，以在近红外（NIR）辐射下实现有效的光动力抗菌活性。排列的基于电纺纤维的膜复合材料(SFCF@Film)表现出良好的机械性能和期望的血液相容性。SFCF@Film为指导细胞取向和促进细胞生长提供了有希望的指导线索。重要的是,SFCF@膜在NIR照射下有效产生ROS以杀死金黄色葡萄球菌，以在10分钟内治疗伤口感染，并促进巨噬细胞的M2极化，以在后期愈合。因此,我们认为这种工程生物支架可以成为处理伤口感染的有力策略。
