Abstract：
Postoperative abdominal adhesions often occur after abdominal surgery; barrier membranes which mimic peritoneal tissue can be constructed to prevent abdominal adhesions. To this end, silk fibroin (SF) sheets were coated with polyvinyl alcohol (PVA) and agarose (AGA) at PVA:AGA ratios of 100:0, 70:30, 50:50, 30:70, and 0:100 to create a composite anti-adhesive barrier and allow us to identify a suitable coating ratio. The membranes were characterized in terms of their molecular organization, structure, and morphology using Fourier transform Infrared spectrometer (FT-IR), differential scanning calorimeter (DSC), and scanning electron microscope (SEM), respectively. The physical and mechanical properties of the membranes and their biological performance (i.e., fibroblast proliferation and invasion) were tested in vitro. Each membrane showed both smooth and rough surface characteristics. Membranes coated with PVA:AGA at ratios of 100:0, 70:30, 50:50, and 30:70 exhibited more -OH and amide III moieties than those coated with 0:100 PVA:AGA, which consequently affected structural organization, degradation, and fibroblast viability. The 0:100 PVA:AGA-coated degraded the fastest. Barrier membranes coated with 100:0 and 70:30 PVA: AGA demonstrated reduced fibroblast proliferation and attachment. The membrane coated with 70:30 PVA:AGA exhibited a stable appearance, and did not curl under wet conditions. Therefore, SF sheets coated with 70:30 PVA:AGA show promise as anti-adhesive barrier membranes for further development.
摘要：
术后腹部粘连通常发生在腹部手术后；可以构建模仿腹膜组织的屏障膜以防止腹部粘连。为此,丝纤蛋白(SF)片用聚乙烯醇(PVA)和琼脂糖(AGA)以100:0、70:30、50:50、30:70和0:100的PVA:AGA比率涂覆,以产生复合抗粘附屏障,并允许我们确定合适的涂覆比率。膜的特征在于它们的分子组织，结构,和形态学使用傅里叶变换红外光谱仪（FT-IR），差示扫描量热仪(DSC),和扫描电子显微镜（SEM），分别。膜的物理和机械性能及其生物学性能（即，成纤维细胞增殖和侵袭）在体外测试。每个膜都显示出光滑和粗糙的表面特征。以100:0、70:30、50:50和30:70的比例涂覆有PVA:AGA的膜比涂覆有0:100PVA:AGA的膜表现出更多的-OH和酰胺III部分，从而影响了结构组织，降解，和成纤维细胞活力。0:100PVA:AGA涂层降解最快。涂覆有100:0和70:30PVA:AGA的屏障膜显示出减少的成纤维细胞增殖和附着。用70:30PVA:AGA涂覆的膜表现出稳定的外观，并且在潮湿条件下没有卷曲。因此,涂覆有70:30PVA:AGA的SF片材显示出作为用于进一步开发的抗粘附屏障膜的前景。
