Abstract：
Multiple sclerosis (MS) is an autoimmune neurodegenerative disease in which the immune system attacks myelin basic protein of nerve axons. Recently, there has been growing interest in studying the role of a newly described population of immunity cells - innate lymphoid cells (ILCs) in the pathogenesis of the disease. At the same time, it was found that during pregnancy there is a weakening of Th1-mediated autoimmune pathologies manifestations, including MS. In this work, we studied phenotypic characteristics of ILC in MS patients in comparison with healthy donors after 48 h incubation with pregnancy hormone estriol (E3) and commensal microflora cells. To activate ILC, strains of Ecsherichia coli K12 and Lactobacillus plantarum 8R-A3 were used. ILC phenotype was assessed by flow cytometry using monoclonal antibody staining. It has been established that E3 and bacterial factors are able to regulate the maturation of ILC subtypes and their cytokines in different ways. In general, the studied factors influence the phenotypic changes in ILC cells, leading to the transition from one type to another, both in healthy donors and in MS patients.
摘要：
多发性硬化症（MS）是一种自身免疫性神经退行性疾病，其中免疫系统攻击神经轴突的髓磷脂碱性蛋白。最近,人们对研究新描述的免疫细胞群-先天性淋巴样细胞（ILC）在疾病发病机理中的作用越来越感兴趣。同时,发现在怀孕期间Th1介导的自身免疫性病变表现减弱，包括女士在这项工作中,我们研究了MS患者与健康供体在妊娠激素雌三醇(E3)和共生微生物区系孵育48小时后ILC的表型特征。要激活ILC，使用大肠杆菌K12和植物乳杆菌8R-A3菌株。使用单克隆抗体染色通过流式细胞术评估ILC表型。已经确定E3和细菌因子能够以不同方式调节ILC亚型及其细胞因子的成熟。总的来说,研究的因素影响ILC细胞的表型变化，导致从一种类型过渡到另一种类型，在健康供体和MS患者中。
