PDF(Pubmed)
Abstract：
Blueberry fruits have been widely explored for their rich composition of bioactive compounds with recognized health benefits. In contrast, blueberry pruning waste (BPW), generated during the pruning stages of blueberries, has been typically overlooked, even though it can represent a potential source of natural antioxidants. This study aims to characterize the value-added compounds extracted from BPW using green techniques, namely microwave-assisted and subcritical water extraction. The total phenolic content ranged from 157 ± 5 to 335 ± 12 mg GAE/g dw, while the radical scavenging activity determined by a DPPH assay varied from 223 ± 21 to 453 ± 21 mg Trolox equivalents/g dw. Additionally, to ensure the safe application of BPW and its extracts, a screening of pesticides and several environmental contaminants was conducted. Chlorpyrifos-methyl was quantified at a concentration of 4.27 µg/kg in a Bluecrop variety collected in 2019; however, none of the studied compounds were found in the extracts. Despite the presence of a pesticide, this level was below the maximum residue limits for blueberry crops. The results of this study demonstrated the potential of this agro-industrial residue as a natural source of bioactive compounds with high antioxidant activity for food industry applications.
摘要：
蓝莓果实因其具有公认的健康益处的生物活性化合物的丰富组成而被广泛探索。相比之下,蓝莓修剪废料（BPW），在蓝莓修剪阶段产生的，通常被忽视，即使它可以代表天然抗氧化剂的潜在来源。本研究旨在使用绿色技术表征从BPW中提取的增值化合物，即微波辅助和亚临界水萃取。总酚含量范围为157±5至335±12mgGAE/gdw，而通过DPPH测定确定的自由基清除活性在223±21至453±21mgTrolox当量/gdw之间变化。此外,为了确保BPW及其提取物的安全应用，对杀虫剂和几种环境污染物进行了筛选。在2019年收集的Bluecrop品种中，甲基毒死蜱的浓度为4.27µg/kg;但是，在提取物中没有发现研究的化合物。尽管有杀虫剂,这一水平低于蓝莓作物的最大残留限值。这项研究的结果表明，这种农业工业残留物具有作为食品工业应用中具有高抗氧化活性的生物活性化合物的天然来源的潜力。
