Abstract：
BACKGROUND: Achyranthes aspera L. (family Amaranthaceae) is a plant species valued in Ayurveda for the treatment of respiratory ailments. Scientific validation of its antiallergic potential was aimed.
RESULTS: Three extracts of A. aspera [aqueous (AaAq), hydroalcoholic (AaHA), ethanolic (AaEt)] were evaluated for their potency against C48/80-induced anaphylaxis in mice at 200 mg/kg BW oral dose. The effective dose of the most potent extract was determined through its effect on C48/80-induced anaphylaxis, and was further analyzed through its effect on mast cell degranulation, histamine-induced bronchospasm and ovalbumin (OVA)-induced asthma in a murine model. Among the three extracts, AaAq was found to be most potent at 200 mg/kg BW. AaAq 400 (400 mg/kg BW) was found to be the most effective dose in terms of inhibition of mortality and histamine level. AaAq 400 prevented the peritoneal and mesenteric mast cells from undergoing morphological changes due to degranulation induced by C48/80. Further, AaAq 400 delayed pre-convulsive time in histamine-induced bronchospasm. In the OVA-induced asthma model, AaAq 400 inhibited the level of inflammatory cell count in blood, bronchoalveolar lavage fluid and peritoneal fluid of mice. The Th2 cytokines (IL-4, IL-5, IL-13), TGF-β and OVA-specific IgE were also reduced as evaluated by ELISA. Also, significant reduction in IL-5 (an eosinophilia indicator) transcript abundance and lung inflammatory score was observed. AaAq was safe up to 4000 mg/kg BW.
CONCLUSIONS: Thus AaAq 400 possesses significant antiallergic potential and acts via attenuation of C48/80-induced anaphylaxis and inhibition of mast cell degranulation. It reduces pre-convulsive dyspnea in histamine-induced bronchospasm and Th2 cytokines in asthmatic mice.
RESULTS: Three extracts of A. aspera [aqueous (AaAq), hydroalcoholic (AaHA), ethanolic (AaEt)] were evaluated for their potency against C48/80-induced anaphylaxis in mice at 200 mg/kg BW oral dose. The effective dose of the most potent extract was determined through its effect on C48/80-induced anaphylaxis, and was further analyzed through its effect on mast cell degranulation, histamine-induced bronchospasm and ovalbumin (OVA)-induced asthma in a murine model. Among the three extracts, AaAq was found to be most potent at 200 mg/kg BW. AaAq 400 (400 mg/kg BW) was found to be the most effective dose in terms of inhibition of mortality and histamine level. AaAq 400 prevented the peritoneal and mesenteric mast cells from undergoing morphological changes due to degranulation induced by C48/80. Further, AaAq 400 delayed pre-convulsive time in histamine-induced bronchospasm. In the OVA-induced asthma model, AaAq 400 inhibited the level of inflammatory cell count in blood, bronchoalveolar lavage fluid and peritoneal fluid of mice. The Th2 cytokines (IL-4, IL-5, IL-13), TGF-β and OVA-specific IgE were also reduced as evaluated by ELISA. Also, significant reduction in IL-5 (an eosinophilia indicator) transcript abundance and lung inflammatory score was observed. AaAq was safe up to 4000 mg/kg BW.
CONCLUSIONS: Thus AaAq 400 possesses significant antiallergic potential and acts via attenuation of C48/80-induced anaphylaxis and inhibition of mast cell degranulation. It reduces pre-convulsive dyspnea in histamine-induced bronchospasm and Th2 cytokines in asthmatic mice.
摘要：
背景：牛膝（Amaranthaceae家族）是阿育吠陀中对呼吸系统疾病的治疗有价值的植物物种。旨在对其抗过敏潜力进行科学验证。
结果：A.aspera的三种提取物[水性（AaAq），水醇（AaHA），乙醇（AaEt）]在200mg/kgBW口服剂量的小鼠中评估了它们对C48/80诱导的过敏反应的效力。最有效的提取物的有效剂量是通过其对C48/80诱导的过敏反应的作用来确定的，并通过其对肥大细胞脱颗粒的影响进一步分析，小鼠模型中组胺诱导的支气管痉挛和卵清蛋白（OVA）诱导的哮喘。在三个摘录中，发现AaAq在200mg/kgBW下最有效。发现AaAq400(400mg/kgBW)在抑制死亡率和组胺水平方面是最有效的剂量。AaAq400可防止腹膜和肠系膜肥大细胞由于C48/80诱导的脱颗粒而发生形态学变化。Further,AaAq400延迟了组胺诱导的支气管痉挛的抽搐前时间。在OVA诱导的哮喘模型中，AaAq400抑制血液中炎症细胞计数的水平，小鼠支气管肺泡灌洗液和腹腔液。Th2细胞因子（IL-4，IL-5，IL-13），如通过ELISA评估的,TGF-β和OVA特异性IgE也降低。此外，观察到IL-5(嗜酸性粒细胞增多指标)转录物丰度和肺部炎症评分显著降低.AaAq在4000mg/kg体重下是安全的。
结论：因此，AaAq400具有显著的抗过敏潜力，并通过减弱C48/80诱导的过敏反应和抑制肥大细胞脱颗粒而发挥作用。它减少了哮喘小鼠中组胺诱导的支气管痉挛和Th2细胞因子的惊厥前呼吸困难。
结果：A.aspera的三种提取物[水性（AaAq），水醇（AaHA），乙醇（AaEt）]在200mg/kgBW口服剂量的小鼠中评估了它们对C48/80诱导的过敏反应的效力。最有效的提取物的有效剂量是通过其对C48/80诱导的过敏反应的作用来确定的，并通过其对肥大细胞脱颗粒的影响进一步分析，小鼠模型中组胺诱导的支气管痉挛和卵清蛋白（OVA）诱导的哮喘。在三个摘录中，发现AaAq在200mg/kgBW下最有效。发现AaAq400(400mg/kgBW)在抑制死亡率和组胺水平方面是最有效的剂量。AaAq400可防止腹膜和肠系膜肥大细胞由于C48/80诱导的脱颗粒而发生形态学变化。Further,AaAq400延迟了组胺诱导的支气管痉挛的抽搐前时间。在OVA诱导的哮喘模型中，AaAq400抑制血液中炎症细胞计数的水平，小鼠支气管肺泡灌洗液和腹腔液。Th2细胞因子（IL-4，IL-5，IL-13），如通过ELISA评估的,TGF-β和OVA特异性IgE也降低。此外，观察到IL-5(嗜酸性粒细胞增多指标)转录物丰度和肺部炎症评分显著降低.AaAq在4000mg/kg体重下是安全的。
结论：因此，AaAq400具有显著的抗过敏潜力，并通过减弱C48/80诱导的过敏反应和抑制肥大细胞脱颗粒而发挥作用。它减少了哮喘小鼠中组胺诱导的支气管痉挛和Th2细胞因子的惊厥前呼吸困难。
