关键词: ELISA Yersinia Yersinia outer membrane protein domestic pig

Mesh : Swine Animals Humans Yersinia Yersinia pseudotuberculosis Sus scrofa Japan / epidemiology Yersinia enterocolitica

来  源:   DOI:10.1292/jvms.23-0032   PDF(Pubmed)

Abstract:
This study aimed to investigate the prevalence of antibodies against pathogenic Yersinia such as Y. enterocolitica and Y. pseudotuberculosis in domestic pigs. A total of 650 serum samples from pigs in nine regions of the Chiba Prefecture in Japan, were tested using plasmid-encoded Yersinia outer membrane protein (Yops) antigen ELISA. The cutoff value was calculated using 20 pathogenic Yersinia-free pig serum samples. According to the cutoff value, 246 (37.8%) pigs from seven regions were considered seropositive for pathogenic Yersinia during the study period. These results indicate that pathogenic Yersinia is widespread in pigs in Chiba, which may become the source of human yersiniosis in this region.
摘要:
本研究旨在调查家猪中针对致病性耶尔森氏菌如小肠结肠炎耶尔森氏菌和假结核耶尔森氏菌的抗体的流行情况。日本千叶县九个地区的猪血清样本共650份,使用质粒编码的耶尔森氏菌外膜蛋白(Yops)抗原ELISA进行测试。使用20个无致病性耶尔森氏菌的猪血清样品计算截断值。根据截止值,在研究期间,来自七个地区的246只(37.8%)猪被认为是致病性耶尔森氏菌的血清阳性。这些结果表明,致病性耶尔森氏菌在千叶猪中普遍存在,这可能成为该地区人类耶尔森氏菌病的源头。
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