Abstract：
Due to the late detection of stomach cancer, this cancer usually causes high mortality. The development of an electrochemical genosensor to measure microRNA 106b (miR-106b), as a gastric cancer biomarker, is the aim of this effort. In this regard, first, 1,3,5-benzenetricarboxylate (BTC) metal-organic frameworks (Zn-BTC MOF) were self-assembled on the glassy carbon electrode and then the probe (ssDNA) was immobilized on it. The morphology Zn-BTC MOF was characterized by SEM, FT-IR, Raman and X-Ray techniques. Zn-BTC MOF as a biosensor substrate has strong interaction with ssDNA. Quantitative measurement of miR-106b was performed by electrochemical impedance spectroscopy (EIS). To perform this measurement, the difference of the charge transfer resistances (ΔRct) of Nyquist plots of the ssDNA probe modified electrode before and after hybridization with miR-106b was obtained and used as an analytical signal. Using the suggested genosensor, it is possible to measure miR-106b in the concentration range of 1.0 fM to 1.0 μM with a detection limit of 0.65 fM under optimal conditions. Moreover, at the genosensor surface, miR-106b can be detected from a non-complementary and a single base mismatch sequence. Also, the genosensor was used to assess miR-106b in a human serum sample and obtained satisfactory results.
摘要：
由于胃癌的发现较晚，这种癌症通常会导致高死亡率。用于测量microRNA106b（miR-106b）的电化学基因传感器的开发，作为胃癌的生物标志物，是这项努力的目的。在这方面,首先,将1,3,5-苯三羧酸盐（BTC）金属有机骨架（Zn-BTCMOF）自组装在玻碳电极上，然后将探针（ssDNA）固定在其上。通过SEM对Zn-BTCMOF的形貌进行了表征,FT-IR,拉曼和X射线技术。Zn-BTCMOF作为生物传感器底物与ssDNA具有很强的相互作用。通过电化学阻抗谱(EIS)进行miR-106b的定量测量。要执行此测量，获得ssDNA探针修饰电极在与miR-106b杂交之前和之后的Nyquist图的电荷转移电阻(ΔRct)的差异并用作分析信号。使用建议的基因传感器，在最佳条件下以0.65fM的检出限在1.0fM至1.0μM的浓度范围内测量miR-106b是可能的。此外，在基因传感器表面，miR-106b可以从非互补和单碱基错配序列中检测到。此外，该基因传感器用于评估人血清样品中的miR-106b,并获得了令人满意的结果.
