PDF(Pubmed)
Abstract：
The genome of a putative Nitrosopumilaceae virus with a hypothetical spindle-shaped particle morphology was identified in the Yangshan Harbour metavirome from the East China Sea through protein similarity comparison and structure analysis. This discovery was accompanied by a set of 10 geographically dispersed close relatives found in the environmental virus datasets from typical locations of ammonia-oxidizing archaeon distribution. Its host prediction was supported by iPHoP prediction and protein sequence similarity. The structure of the predicted major capsid protein, together with the overall N-glycosylation site, the transmembrane helices prediction, the hydrophilicity profile, and the docking simulation of the major capsid proteins, indicate that these viruses resemble spindle-shaped viruses. It suggests a similarly assembled structure and, consequently, a possibly spindle-shaped morphology of these newly discovered archaeal viruses.
摘要：
通过蛋白质相似性比较和结构分析，在东海洋山港metavirome中鉴定了具有假想纺锤形颗粒形态的推定的亚硝科病毒的基因组。这一发现伴随着一组10个地理上分散的近亲，这些近亲在环境病毒数据集中来自氨氧化古菌分布的典型位置。其宿主预测得到iPHoP预测和蛋白质序列相似性的支持。预测的主要衣壳蛋白的结构，连同整个N-糖基化位点，跨膜螺旋预测，亲水性特征，以及主要衣壳蛋白的对接模拟，表明这些病毒类似于纺锤形病毒。它提出了一个类似的组装结构，因此,这些新发现的古细菌病毒的可能的纺锤形形态。
