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Abstract：
African swine fever (ASF) is a highly contagious disease caused by African swine fever virus (ASFV), affecting domestic and wild boars. The polyprotein pp220 of ASFV is responsible for producing the major structural proteins p150, p37, p14, p34, and p5 via proteolytic processing. The p34 protein is the main component of the ASFV core shell. However, the immunologic properties of the p34 protein in vitro and in vivo remain unclear. The results showed that the recombinant p34 protein expressed in prokaryotes and eukaryotes could react with convalescent swine sera to ASFV, suggesting that p34 is an immunogenic protein. Significantly, anti-p34 antibodies were found to inhibit the replication of ASFV in target cells. Furthermore, rabbits immunized with the recombinant C-strain of classical swine fever virus containing p34 produced both anti-p34 humoral and cellular immune responses. In addition, the p34 protein could induce a cell-mediated immune response, and a T-cell epitope on the p34 protein was identified using immunoinformatics and enzyme-linked immunospot (ELIspot) assay. Our study demonstrates that the p34 protein is a novel antigen of ASFV with protective potential.
摘要：
非洲猪瘟（ASF）是由非洲猪瘟病毒（ASFV）引起的高度传染性疾病，影响家猪和野猪。ASFV的多蛋白pp220负责通过蛋白水解加工产生主要结构蛋白p150、p37、p14、p34和p5。p34蛋白是ASFV核壳的主要成分。然而,p34蛋白在体外和体内的免疫特性仍不清楚。结果表明，原核生物和真核生物表达的重组p34蛋白可与恢复期猪血清反应，表明p34是一种免疫原性蛋白。重要的是,发现抗p34抗体抑制ASFV在靶细胞中的复制.此外，用含有p34的重组猪瘟病毒C株免疫的兔子产生了抗p34的体液和细胞免疫反应。此外,p34蛋白可以诱导细胞介导的免疫反应，使用免疫信息学和酶联免疫斑点(ELIspot)测定鉴定p34蛋白上的T细胞表位。我们的研究表明，p34蛋白是一种具有保护潜力的新型ASFV抗原。
