PDF(Pubmed)
Abstract：
(1) Background: Group A rotaviruses (RVAs) are the primary cause of severe intestinal diseases in piglets. Porcine rotaviruses (PoRVs) are widely prevalent in Chinese farms, resulting in significant economic losses to the livestock industry. However, isolation of PoRVs is challenging, and their pathogenicity in piglets is not well understood. (2) Methods: We conducted clinical testing on a farm in Jiangsu Province, China, and isolated PoRV by continuously passaging on MA104 cells. Subsequently, the pathogenicity of the isolated strain in piglets was investigated. The piglets of the PoRV-infection group were orally inoculated with 1 mL of 1.0 × 106 TCID50 PoRV, whereas those of the mock-infection group were fed with an equivalent amount of DMEM. (3) Results: A G5P[23] genotype PoRV strain was successfully isolated from one of the positive samples and named RVA/Pig/China/JS/2023/G5P[23](JS). The genomic constellation of this strain was G5-P[23]-I5-R1-C1-M1-A8-N1-T1-E1-H1. Sequence analysis revealed that the genes VP3, VP7, NSP2, and NSP4 of the JS strain were closely related to human RVAs, whereas the remaining gene segments were closely related to porcine RVAs, indicating a reassortment between porcine and human strains. Furthermore, infection of 15-day-old piglets with the JS strain resulted in a diarrheal rate of 100% (8 of 8) and a mortality rate of 37.5% (3 of 8). (4) Conclusions: The isolated G5P[23] genotype rotavirus strain, which exhibited strong pathogenicity in piglets, may have resulted from recombination between porcine and human strains. It may serve as a potential candidate strain for developing vaccines, and its immunogenicity can be tested in future studies.
摘要：
(1)背景:A组轮状病毒(RVA)是仔猪严重肠道疾病的主要病因。猪轮状病毒（PoRVs）在中国农场广泛流行，给畜牧业造成重大经济损失。然而,隔离PoRV具有挑战性，它们在仔猪中的致病性尚不清楚。(2)方法:在江苏省某农场进行临床检验,中国,并通过在MA104细胞上连续传代来分离PoRV。随后，研究了分离菌株对仔猪的致病性。PoRV感染组仔猪口服接种1.0×106TCID50PoRV1mL,而模拟感染组的那些被喂食等量的DMEM。（3）结果：从其中一个阳性样品中成功分离出G5P[23]基因型PoRV菌株，命名为RVA/Pig/China/JS/2023/G5P[23]（JS）。该菌株的基因组星座是G5-P[23]-I5-R1-C1-M1-A8-N1-T1-E1-H1。序列分析表明，JS菌株的基因VP3，VP7，NSP2和NSP4与人RVA密切相关，而其余基因片段与猪RVA密切相关，表明猪和人菌株之间的重新分类。此外，用JS菌株感染15日龄仔猪导致腹泻率为100％（8个中的8个），死亡率为37.5％（8个中的3个）。(4)结论:G5P[23]基因型轮状病毒分离株,在仔猪身上表现出很强的致病性，可能是由猪和人菌株之间的重组引起的。它可能是开发疫苗的潜在候选菌株，其免疫原性可以在未来的研究中进行测试。
