PDF(Pubmed)
Abstract：
OBJECTIVE: To investigate the role of Dkk1 as a predictor of response to NACT in BC patients.
METHODS: This retrospective monocentric study included 145 women who had undergone NACT followed by breast surgery. Dkk1 protein expression was assessed using immunohistochemistry staining in core needle biopsies and mammary carcinoma specimens.
RESULTS: Dkk1 levels were lower in treated BC tumours than in untreated tumours. The outcomes of 68 matched pre- and post-therapy tissues showed that Dkk1 levels in mammary carcinoma tissues were significantly predicted by levels in core needle biopsies and that Dkk1 expression was reduced in 83% of cases. Smaller cT stage, positive Her2 expression, and decreased Dkk1-IRS in core needle biopsy tissues were all independent predictors of regression grade (R4), according to Sinn. However, the percentage of Dkk1 expression differences prior to and following NACT had no effect on PFS or OS.
CONCLUSIONS: In this study, we demonstrated for the first time that Dkk1 could be identified as an independent predictor of NACT response in BC patients, particularly those with TNBC. Further research with a multicentric expanded (pre-/post-therapy) sample set and better-defined populations in terms of molecular subtypes, therapy modality, and long-term follow-up is recommended to obtain more solid evidence.
METHODS: This retrospective monocentric study included 145 women who had undergone NACT followed by breast surgery. Dkk1 protein expression was assessed using immunohistochemistry staining in core needle biopsies and mammary carcinoma specimens.
RESULTS: Dkk1 levels were lower in treated BC tumours than in untreated tumours. The outcomes of 68 matched pre- and post-therapy tissues showed that Dkk1 levels in mammary carcinoma tissues were significantly predicted by levels in core needle biopsies and that Dkk1 expression was reduced in 83% of cases. Smaller cT stage, positive Her2 expression, and decreased Dkk1-IRS in core needle biopsy tissues were all independent predictors of regression grade (R4), according to Sinn. However, the percentage of Dkk1 expression differences prior to and following NACT had no effect on PFS or OS.
CONCLUSIONS: In this study, we demonstrated for the first time that Dkk1 could be identified as an independent predictor of NACT response in BC patients, particularly those with TNBC. Further research with a multicentric expanded (pre-/post-therapy) sample set and better-defined populations in terms of molecular subtypes, therapy modality, and long-term follow-up is recommended to obtain more solid evidence.
摘要：
目的：研究Dkk1作为BC患者对NACT反应的预测因子的作用。
方法：这项回顾性单中心研究包括145名接受NACT术后乳房手术的妇女。在核心针活检和乳腺癌标本中使用免疫组织化学染色评估Dkk1蛋白的表达。
结果:Dkk1水平在治疗的BC肿瘤中低于在未治疗的肿瘤中。68个匹配的治疗前和治疗后组织的结果表明，乳腺癌组织中的Dkk1水平可通过芯针活检中的水平显着预测，而83％的病例中Dkk1表达降低。较小的cT阶段，Her2阳性表达，芯针活检组织中Dkk1-IRS的降低是回归等级（R4）的独立预测因子，根据Sinn然而,NACT前后Dkk1表达差异百分比对PFS或OS无影响.
结论：在这项研究中，我们首次证明Dkk1可作为BC患者NACT反应的独立预测因子,特别是那些与TNBC。在分子亚型方面，使用多中心扩展（治疗前/治疗后）样本集和更好定义的群体进行进一步研究，治疗方式，建议长期随访以获得更确凿的证据。
方法：这项回顾性单中心研究包括145名接受NACT术后乳房手术的妇女。在核心针活检和乳腺癌标本中使用免疫组织化学染色评估Dkk1蛋白的表达。
结果:Dkk1水平在治疗的BC肿瘤中低于在未治疗的肿瘤中。68个匹配的治疗前和治疗后组织的结果表明，乳腺癌组织中的Dkk1水平可通过芯针活检中的水平显着预测，而83％的病例中Dkk1表达降低。较小的cT阶段，Her2阳性表达，芯针活检组织中Dkk1-IRS的降低是回归等级（R4）的独立预测因子，根据Sinn然而,NACT前后Dkk1表达差异百分比对PFS或OS无影响.
结论：在这项研究中，我们首次证明Dkk1可作为BC患者NACT反应的独立预测因子,特别是那些与TNBC。在分子亚型方面，使用多中心扩展（治疗前/治疗后）样本集和更好定义的群体进行进一步研究，治疗方式，建议长期随访以获得更确凿的证据。
