Abstract：
A high-throughput surface-enhanced Raman scattering (SERS)-sensing platform is presented for FNT detection in human urine without any sample preparation. The sensing platform is based on plasmonics-active silver-coated sharply branched gold nanostars (SGNS). The effect of silver thickness was investigated experimentally and theoretically, and the results indicated that SERS enhancement was maximum at an optimum silver thickness of 45 nm on the sharply spiked SGNS. The proposed high-throughput SERS platform exhibited ultrahigh sensitivity and excellent enhancement uniformity for a model analyte, i.e., crystal violet. Moreover, the SERS-sensing platform demonstrated good sensitivity of FNT spiked in human urine samples with two differential linear response ranges of 2 to 0.2 µg/mL and 0.1 µg/mL to 100 pg/mL, respectively, with a detection limit as low as 10.02 pg/mL. The spiked human urine samples show satisfactory recovery values from 92.5 to 102% with relative standard deviations (RSD) of less than 10%. In summary, the high-throughput performance of the proposed microplate-based SERS platform demonstrated great potential for rapid low-cost SERS-based sensing applications.
摘要：
提出了一种高通量表面增强拉曼散射（SERS）传感平台，用于人体尿液中的FNT检测，无需任何样品制备。传感平台基于等离子体活性银涂层的尖锐分支金纳米星（SGNS）。对银厚度的影响进行了实验和理论研究,结果表明，在急剧加标的SGNS上，在45nm的最佳银厚度下，SERS增强最大。提出的高通量SERS平台对模型分析物表现出超高灵敏度和优异的增强均匀性，即，结晶紫.此外，SERS传感平台显示了人尿液样品中掺入FNT的良好灵敏度，具有2至0.2µg/mL和0.1µg/mL至100pg/mL的两个差分线性响应范围，分别,检测限低至10.02pg/mL。加标的人尿液样品显示出92.5至102%的令人满意的回收率值,相对标准偏差(RSD)小于10%。总之,所提出的基于微孔板的SERS平台的高通量性能显示出快速低成本的基于SERS的传感应用的巨大潜力。
