PDF(Pubmed)
Abstract：
UNASSIGNED: Ultrasensitive bacterial detection methods are crucial to ensuring accurate diagnosis and effective clinical monitoring, given the significant threat bacterial infections pose to human health. The aim of this study is to develop a biosensor with capabilities for broad-spectrum bacterial detection, rapid processing, and cost-effectiveness.
UNASSIGNED: A magnetically-assisted SERS biosensor was designed, employing wheat germ agglutinin (WGA) for broad-spectrum recognition and antibodies for specific capture. Gold nanostars (AuNSs) were sequentially modified with the Raman reporter molecules and WGA, creating a versatile SERS tag with high affinity for a diverse range of bacteria. Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) antibody-modified Fe3O4 magnetic gold nanoparticles (MGNPs) served as the capture probes. Target bacteria were captured by MGNPs and combined with SERS tags, forming a \"sandwich\" composite structure for bacterial detection.
UNASSIGNED: AuNSs, with a core size of 65 nm, exhibited excellent storage stability (RSD=5.6%) and demonstrated superior SERS enhancement compared to colloidal gold nanoparticles. Efficient binding of S. aureus and P. aeruginosa to MGNPs resulted in capture efficiencies of 89.13% and 85.31%, respectively. Under optimized conditions, the developed assay achieved a limit of detection (LOD) of 7 CFU/mL for S. aureus and 5 CFU/mL for P. aeruginosa. The bacterial concentration (10-106 CFU/mL) showed a strong linear correlation with the SERS intensity at 1331 cm-1. Additionally, high recoveries (84.8% - 118.0%) and low RSD (6.21% - 11.42%) were observed in spiked human urine samples.
UNASSIGNED: This study introduces a simple and innovative magnetically-assisted SERS biosensor for the sensitive and quantitative detection of S. aureus or P. aeruginosa, utilizing WGA and antibodies. The developed biosensor enhances the capabilities of the \"sandwich\" type SERS biosensor, offering a novel and effective platform for accurate and timely clinical diagnosis of bacterial infections.
UNASSIGNED: A magnetically-assisted SERS biosensor was designed, employing wheat germ agglutinin (WGA) for broad-spectrum recognition and antibodies for specific capture. Gold nanostars (AuNSs) were sequentially modified with the Raman reporter molecules and WGA, creating a versatile SERS tag with high affinity for a diverse range of bacteria. Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) antibody-modified Fe3O4 magnetic gold nanoparticles (MGNPs) served as the capture probes. Target bacteria were captured by MGNPs and combined with SERS tags, forming a \"sandwich\" composite structure for bacterial detection.
UNASSIGNED: AuNSs, with a core size of 65 nm, exhibited excellent storage stability (RSD=5.6%) and demonstrated superior SERS enhancement compared to colloidal gold nanoparticles. Efficient binding of S. aureus and P. aeruginosa to MGNPs resulted in capture efficiencies of 89.13% and 85.31%, respectively. Under optimized conditions, the developed assay achieved a limit of detection (LOD) of 7 CFU/mL for S. aureus and 5 CFU/mL for P. aeruginosa. The bacterial concentration (10-106 CFU/mL) showed a strong linear correlation with the SERS intensity at 1331 cm-1. Additionally, high recoveries (84.8% - 118.0%) and low RSD (6.21% - 11.42%) were observed in spiked human urine samples.
UNASSIGNED: This study introduces a simple and innovative magnetically-assisted SERS biosensor for the sensitive and quantitative detection of S. aureus or P. aeruginosa, utilizing WGA and antibodies. The developed biosensor enhances the capabilities of the \"sandwich\" type SERS biosensor, offering a novel and effective platform for accurate and timely clinical diagnosis of bacterial infections.
摘要：
■超灵敏细菌检测方法对于确保准确诊断和有效的临床监测至关重要，考虑到细菌感染对人类健康构成的重大威胁。这项研究的目的是开发一种具有广谱细菌检测功能的生物传感器，快速加工,和成本效益。
■设计了一种磁辅助SERS生物传感器，使用小麦胚芽凝集素（WGA）进行广谱识别，并使用抗体进行特异性捕获。金纳米星（AuNSs）依次用拉曼报道分子和WGA修饰，创建一个通用的SERS标签与多种细菌的高亲和力。金黄色葡萄球菌（S。金黄色葡萄球菌)和铜绿假单胞菌(P.铜绿假）抗体修饰的Fe3O4磁性金纳米颗粒（MGNPs）用作捕获探针。通过MGNPs捕获目标细菌，并结合SERS标签，形成用于细菌检测的“三明治”复合结构。
■AuNS，核心尺寸为65纳米，与胶体金纳米颗粒相比,表现出优异的储存稳定性(RSD=5.6%),并且表现出优异的SERS增强。金黄色葡萄球菌和铜绿假单胞菌与MGNP的有效结合导致89.13%和85.31%的捕获效率,分别。在优化条件下，该方法对金黄色葡萄球菌和铜绿假单胞菌分别达到7CFU/mL和5CFU/mL的检测限(LOD).细菌浓度（10-106CFU/mL）与1331cm-1处的SERS强度具有很强的线性相关性。此外,在加标的人尿液样品中观察到高回收率（84.8％-118.0％）和低RSD（6.21％-11.42％）。
■这项研究介绍了一种简单且创新的磁辅助SERS生物传感器，用于敏感和定量检测金黄色葡萄球菌或铜绿假单胞菌，利用WGA和抗体。开发的生物传感器增强了“三明治”型SERS生物传感器的功能，为细菌感染的准确和及时的临床诊断提供了一个新颖和有效的平台。
■设计了一种磁辅助SERS生物传感器，使用小麦胚芽凝集素（WGA）进行广谱识别，并使用抗体进行特异性捕获。金纳米星（AuNSs）依次用拉曼报道分子和WGA修饰，创建一个通用的SERS标签与多种细菌的高亲和力。金黄色葡萄球菌（S。金黄色葡萄球菌)和铜绿假单胞菌(P.铜绿假）抗体修饰的Fe3O4磁性金纳米颗粒（MGNPs）用作捕获探针。通过MGNPs捕获目标细菌，并结合SERS标签，形成用于细菌检测的“三明治”复合结构。
■AuNS，核心尺寸为65纳米，与胶体金纳米颗粒相比,表现出优异的储存稳定性(RSD=5.6%),并且表现出优异的SERS增强。金黄色葡萄球菌和铜绿假单胞菌与MGNP的有效结合导致89.13%和85.31%的捕获效率,分别。在优化条件下，该方法对金黄色葡萄球菌和铜绿假单胞菌分别达到7CFU/mL和5CFU/mL的检测限(LOD).细菌浓度（10-106CFU/mL）与1331cm-1处的SERS强度具有很强的线性相关性。此外,在加标的人尿液样品中观察到高回收率（84.8％-118.0％）和低RSD（6.21％-11.42％）。
■这项研究介绍了一种简单且创新的磁辅助SERS生物传感器，用于敏感和定量检测金黄色葡萄球菌或铜绿假单胞菌，利用WGA和抗体。开发的生物传感器增强了“三明治”型SERS生物传感器的功能，为细菌感染的准确和及时的临床诊断提供了一个新颖和有效的平台。
