PDF(Pubmed)
Abstract：
Fish is a nutritionally rich product; however, it is easily contaminated by pathogenic microorganisms, such as Salmonella spp. Therefore, this study aimed to identify the best concentration of sodium hypochlorite (NaClO), exposure time, and water temperature that allow the most effective antimicrobial effect on the viable population of Salmonella spp. Thus, Salmonella Enteritidis ATCC 13076 and Salmonella Schwarzengrund were exposed to different time frames, ranging from 5 min to 38.5 min, temperatures between 5 and 38.5 °C, and NaClO concentrations ranging from 0.36 to 6.36 ppm, through a central composite rotational design experiment (CCRD). The results demonstrated that the ATCC strain exhibited a quadratic response to sodium hypochlorite when combined with exposure time, indicating that initial contact would already be sufficient for the compound\'s action to inhibit the growth of the mentioned bacteria. However, for S. Schwarzengrund (isolated directly from fish cultivated in aquaculture), both NaClO concentration and exposure time significantly influenced inactivation, following a linear pattern. This suggests that increasing the exposure time of NaClO could be an alternative to enhance Salmonella elimination rates in fish slaughterhouses. Thus, the analysis indicates that the Salmonella spp. strains used in in vitro experiments were sensitive to concentrations equal to or greater than the recommended ones, requiring a longer exposure time combined with the recommended NaClO concentration in the case of isolates from aquaculture.
摘要：
鱼是一种营养丰富的产品；然而，它很容易被病原微生物污染，如沙门氏菌。因此,本研究旨在确定次氯酸钠（NaClO）的最佳浓度，曝光时间,和水温，可以对沙门氏菌的活菌群产生最有效的抗菌作用。因此，肠炎沙门氏菌ATCC13076和Schwarzengrund沙门氏菌暴露于不同的时间范围，从5分钟到38.5分钟，温度介于5和38.5°C之间，和NaClO浓度范围从0.36到6.36ppm，通过中心复合材料旋转设计实验(CCRD)。结果表明，当与暴露时间结合时，ATCC菌株对次氯酸钠表现出二次响应，表明最初的接触已经足以使化合物的作用抑制上述细菌的生长。然而,对于S.Schwarzengrund（直接从水产养殖的鱼类中分离出来），NaClO浓度和暴露时间均显着影响失活，遵循线性模式。这表明增加NaClO的暴露时间可能是提高鱼类屠宰场中沙门氏菌消除率的替代方法。因此，分析表明沙门氏菌属。体外实验中使用的菌株对等于或大于推荐浓度的浓度敏感，在水产养殖分离株的情况下，需要更长的暴露时间和推荐的NaClO浓度。
