Abstract：
Cancer represents a global threat to human health, and effective strategies for improved cancer early diagnosis and treatment are urgently needed. The detection of tumor biomarkers has been one of the important auxiliary means for tumor screening and diagnosis. Mitochondria are crucial subcellular organelles that produce most chemical energy used by cells, control metabolic processes, and maintain cell function. Evidence suggests the close involvement of mitochondria with cancer development. As a consequence, the identification of cancer-associated biomarker expression levels in mitochondria holds significant importance in the diagnosis of early-stage diseases and the monitoring of therapy efficacy. Small-molecule fluorescent probes are effective for the identification and visualization of bioactive entities within biological systems, owing to their heightened sensitivity, expeditious non-invasive analysis and real-time detection capacities. The design principles and sensing mechanisms of mitochondrial targeted fluorescent probes are summarized in this review. Additionally, the biomedical applications of these probes for detecting cancer-associated biomarkers are highlighted. The limitations and challenges of fluorescent probes in vivo are also considered and some future perspectives are provided. This review is expected to provide valuable insights for the future development of novel fluorescent probes for clinical imaging, thereby contributing to the advancement of cancer diagnosis and treatment.
摘要：
癌症是对人类健康的全球性威胁，迫切需要改善癌症早期诊断和治疗的有效策略。肿瘤标志物的检测一直是肿瘤筛查和诊断的重要辅助手段之一。线粒体是至关重要的亚细胞细胞器，产生细胞使用的大部分化学能，控制代谢过程，维持细胞功能。证据表明线粒体与癌症的发展密切相关。因此,线粒体中癌症相关生物标志物表达水平的鉴定对于早期疾病诊断和治疗效果监测具有重要意义.小分子荧光探针对于生物系统内生物活性实体的识别和可视化是有效的，由于他们高度的敏感性，快速的非侵入性分析和实时检测能力。本文综述了线粒体靶向荧光探针的设计原理和传感机制。此外,重点介绍了这些探针在检测癌症相关生物标志物中的生物医学应用.还考虑了体内荧光探针的局限性和挑战，并提供了一些未来的观点。这篇综述有望为未来用于临床成像的新型荧光探针的开发提供有价值的见解。从而促进癌症的诊断和治疗。
