关键词: Escherichia fergusonii Japan heat-labile enterotoxin retail chicken meat whole-genome sequencing

Mesh : Animals Escherichia coli / genetics Enterotoxins / genetics Chickens Japan Hot Temperature Plasmids / genetics Meat Anti-Bacterial Agents / pharmacology Drug Resistance, Bacterial Escherichia

来  源:   DOI:10.1111/1348-0421.13115

Abstract:
This study aimed to reveal the prevalence of heat-labile enterotoxin (LT) gene-positive Escherichia fergusonii in retail chicken meat and genetically characterize these strains. E. fergusonii harboring LT gene was isolated from 6 out of 60 (10%) retail chicken samples in Okinawa, Japan. Whole-genome sequencing analysis revealed that LT gene-positive E. fergusonii from chicken meat and feces contain an IncFII plasmid harboring elt1AB, and suggested to spread clonally to retail chicken through fecal contamination. Additionally, it was found that these strains harbor multidrug-resistant genes on their plasmids. Their pathogenicity and continuous monitoring are required for confirmation.
摘要:
这项研究旨在揭示零售鸡肉中不耐热肠毒素(LT)基因阳性的大肠杆菌的患病率,并对这些菌株进行遗传表征。从冲绳60个零售鸡肉样本中的6个(10%)中分离出带有LT基因的E.Fergusonii,日本。全基因组测序分析显示,来自鸡肉和粪便的LT基因阳性E.fergusonii含有一个含有elt1AB的IncFII质粒,并建议通过粪便污染无性繁殖到零售鸡肉上。此外,发现这些菌株在其质粒上带有多药耐药基因。它们的致病性和持续监测需要确认。
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