Abstract：
The Asian tiger mosquito, Aedes albopictus, is an important vector of chikungunya, dengue, yellow fever, and Zika viruses. Vector control remains an important means for the prevention and control of vector-borne diseases. The development of insecticide resistance has become a serious threat to the efficacy of insecticide-based control programs. To understand the resistance status and the underlying genetic mechanism in mosquitoes in Guangyuan City of Sichuan Province, China, we investigated the susceptibility of Ae. albopictus to four commonly used insecticides. We found that all the examined populations were susceptible to malathion and propoxur. However, Ae. albopictus populations in Guangyuan showed a possible resistance to the two tested pyrethroids (beta-cypermethrin and deltamethrin). Notably, phenotypic resistance to deltamethrin was detected in 2 of the 7 populations. The potential of resistance to pyrethroids was confirmed by the presence of knockdown resistance (kdr) related mutations in the voltage-gated sodium channel. Four kdr mutations (V1016G, I1532T, F1534L, and F1534S) were identified to be present alone or in combination, and their distribution displayed significant spatial heterogeneity. These findings are helpful for making evidence-based mosquito control strategies and highlight the need to regularly monitor the dynamics of pyrethroid resistance in this city.
摘要：
亚洲虎蚊子，白纹伊蚊,是基孔肯雅热的重要矢量,登革热,黄热病,和寨卡病毒。媒介控制仍然是预防和控制媒介传播疾病的重要手段。杀虫剂抗性的发展已严重威胁到基于杀虫剂的控制计划的功效。了解四川省广元市蚊虫的抗性状况及其遗传机制,中国,我们调查了Ae的易感性。白纹以四种常用杀虫剂为主。我们发现所有被检查的人群都对马拉硫磷和丙氧磷敏感。然而,Ae.广元市的白纹伊蚊种群可能对两种测试的拟除虫菊酯（氯氰菊酯和溴氰菊酯）具有抗性。值得注意的是,在7个种群中的2个中检测到对溴氰菊酯的表型抗性。通过电压门控钠通道中存在敲低抗性（kdr）相关突变，证实了对拟除虫菊酯的抗性潜力。四种kdr突变(V1016G，I1532T,F1534L,和F1534S)被鉴定为单独或组合存在，其分布表现出显著的空间异质性。这些发现有助于制定基于证据的蚊子控制策略，并强调需要定期监测该市拟除虫菊酯抗性的动态。
