Abstract：
Degradation of trans-cinnamaldehyde and limonene in cucumber was evaluated under laboratory and greenhouse conditions. Two commercial biopesticides, one based on cinnamon extract and other from orange oil, were utilized. Compound degradation was monitored using gas chromatography (GC) and ultra-high-performance liquid chromatography (UHPLC) coupled to a quadrupole-high-resolution mass analyzer (Q-Orbitrap). In both studies, trans-cinnamaldehyde followed a second-order degradation kinetics, whereas limonene followed a first-order kinetics. The half-life values (DT50 or t1/2) for trans-cinnamaldehyde ranged from 2.02 to 2.49 h, while for limonene this value ranged from 0.49 to 6.17 h. Non-targeted analysis (suspect and unknown modes) allowed for the detection of trans-cinnamaldehyde and limonene metabolites. Benzyl alcohol, cinnamyl alcohol, cinnamic acid, p-tolylacetic acid and 4-hydoxycinnamic acid were tentatively identified as trans-cinnamaldehyde metabolites. While three limonene metabolites, carvone, limonene-1,2-epoxide, and perillyl alcohol, were tentatively identified. Greenhouse studies have not revealed any metabolites of these compounds because the parent compounds degrade more quickly.
摘要：
在实验室和温室条件下评估了黄瓜中反式肉桂醛和柠檬烯的降解。两种商业生物农药，一种基于肉桂提取物和其他来自橙油，被利用。使用与四极杆高分辨率质量分析仪(Q-Orbitrap)偶联的气相色谱(GC)和超高效液相色谱(UHPLC)监测化合物降解。在两项研究中，反式肉桂醛遵循二级降解动力学，而柠檬烯遵循一级动力学。反式肉桂醛的半衰期值(DT50或t1/2)为2.02-2.49h,而柠檬烯的该值范围为0.49至6.17h。非靶向分析（可疑和未知模式）允许检测反式肉桂醛和柠檬烯代谢物。苯甲醇,肉桂醇,肉桂酸,对甲苯乙酸和4-羟基肉桂酸被初步鉴定为反式肉桂醛代谢产物。虽然三种柠檬烯代谢物，Carvone,柠檬烯-1,2-环氧化物，和紫苏醇，暂时确定。温室研究没有发现这些化合物的任何代谢物，因为母体化合物降解更快。
