Abstract：
Inspired by membrane structure of breast milk and infant formula fat globules, four liposomes with different particle size (large and small) and compositions (Single phospholipids contained phosphatidylcholine, complex phospholipids contained phosphatidylcholine, phosphatidylethanolamine and sphingomyelin) were fabricated to deliver lactoferrin and DHA. In vitro infant semi-dynamic digestive behavior and absorption in intestinal organoids of liposomes were investigated. Liposomal structures were negligible changed during semi-dynamic gastric digestion while damaged in intestine. Liposomal degradation rate was primarily influenced by particle size, and complex phospholipids accelerated DHA hydrolysis. The release rate of DHA (91.7 ± 1.3 %) in small-sized liposomes (0.181 ± 0.001 μm) was higher than free DHA (unencapsulated, 64.6 ± 3.4 %). Complex phospholipids liposomal digesta exhibited higher transport efficiency (3.4-fold for fatty acids and 2.0-fold for amino acids) and better organoid growth than digesta of bare nutrients. This study provided new insights into membrane structure-functionality relationship of liposomes and may aid in the development of novel infant nutrient carriers.
摘要：
灵感来自母乳和婴儿配方脂肪球的膜结构，四种具有不同粒径（大小）和组成的脂质体（单磷脂含有磷脂酰胆碱，复杂的磷脂含有磷脂酰胆碱，制造磷脂酰乙醇胺和鞘磷脂）以递送乳铁蛋白和DHA。研究了体外婴儿半动态消化行为和脂质体在肠道类器官中的吸收。在半动态胃消化过程中，脂质体结构的变化可忽略不计，而在肠道中受损。脂质体降解速率主要受粒径影响，和复杂的磷脂加速DHA水解。小尺寸脂质体（0.181±0.001μm）中DHA的释放率（91.7±1.3％）高于游离DHA（未包封，64.6±3.4%)。复合磷脂脂质体消化液比裸营养素消化液具有更高的转运效率（脂肪酸为3.4倍，氨基酸为2.0倍）和更好的类器官生长。这项研究为脂质体的膜结构-功能关系提供了新的见解，并可能有助于新型婴儿营养载体的开发。
