PDF(Pubmed)
Abstract：
UNASSIGNED: Oocyte and embryo quality differs significantly among individuals. Follicular fluid (FF) is a solo environment of oocyte maturation and may flux into the oviduct. Supplementation of in vitro maturation (IVM) and culture (IVC) medium with extracellular vesicles of FFs supports oocyte maturation and embryonic development. We addressed a hypothesis that miRNA profiles in FFs are crucial background of oocyte maturation and embryonic development.
UNASSIGNED: FFs were collected from the ovaries of individual cows, and the FFs were classified into Good or Poor FF based on the developmental rate to the blastocyst stage of enclosed oocytes. miRNAs associated with the Good FFs were explored using small RNA sequencing. In addition, FFs were classified using the concentration of Good-FF-associated miRNAs. These classified FFs or miRNA were added to the IVM or IVC mediums.
UNASSIGNED: Supplementation of IVM and IVC medium with Good FF improved embryonic development. Good FFs contained miR-151-3p and miR-425-5p at a high concentration compared with those in Poor FFs. FFs selected by the concentration of miR-151-3p and miR-425-5p improved oocyte maturation and embryonic development. Supplementation of IVM or IVC medium with either miR-151-3p or miR-425-5p improved embryonic development to the blastocyst stage.
UNASSIGNED: miRNAs were associated with the Good FFs determined oocyte maturation and embryonic development.
UNASSIGNED: FFs were collected from the ovaries of individual cows, and the FFs were classified into Good or Poor FF based on the developmental rate to the blastocyst stage of enclosed oocytes. miRNAs associated with the Good FFs were explored using small RNA sequencing. In addition, FFs were classified using the concentration of Good-FF-associated miRNAs. These classified FFs or miRNA were added to the IVM or IVC mediums.
UNASSIGNED: Supplementation of IVM and IVC medium with Good FF improved embryonic development. Good FFs contained miR-151-3p and miR-425-5p at a high concentration compared with those in Poor FFs. FFs selected by the concentration of miR-151-3p and miR-425-5p improved oocyte maturation and embryonic development. Supplementation of IVM or IVC medium with either miR-151-3p or miR-425-5p improved embryonic development to the blastocyst stage.
UNASSIGNED: miRNAs were associated with the Good FFs determined oocyte maturation and embryonic development.
摘要：
■卵母细胞和胚胎质量在个体之间存在显著差异。卵泡液（FF）是卵母细胞成熟的单独环境，可能会流入输卵管。用FF的细胞外囊泡补充体外成熟（IVM）和培养（IVC）培养基支持卵母细胞成熟和胚胎发育。我们提出了一个假设，即FF中的miRNA谱是卵母细胞成熟和胚胎发育的关键背景。
■从个别母牛的卵巢中收集FF，根据封闭卵母细胞的胚泡期的发育速率，将FF分为好FF或差FF。使用小RNA测序探索与良好FF相关的miRNA。此外,使用Good-FF相关miRNA的浓度对FF进行分类。将这些分类的FF或miRNA添加到IVM或IVC培养基中。
■用良好的FF补充IVM和IVC培养基改善了胚胎发育。与不良FF中的那些相比,良好FF含有高浓度的miR-151-3p和miR-425-5p。由miR-151-3p和miR-425-5p的浓度挑选的FFs增进卵母细胞成熟和胚胎发育。用miR-151-3p或miR-425-5p补充IVM或IVC培养基改善胚胎发育至胚泡期。
■miRNA与决定卵母细胞成熟和胚胎发育的良好FF相关。
■从个别母牛的卵巢中收集FF，根据封闭卵母细胞的胚泡期的发育速率，将FF分为好FF或差FF。使用小RNA测序探索与良好FF相关的miRNA。此外,使用Good-FF相关miRNA的浓度对FF进行分类。将这些分类的FF或miRNA添加到IVM或IVC培养基中。
■用良好的FF补充IVM和IVC培养基改善了胚胎发育。与不良FF中的那些相比,良好FF含有高浓度的miR-151-3p和miR-425-5p。由miR-151-3p和miR-425-5p的浓度挑选的FFs增进卵母细胞成熟和胚胎发育。用miR-151-3p或miR-425-5p补充IVM或IVC培养基改善胚胎发育至胚泡期。
■miRNA与决定卵母细胞成熟和胚胎发育的良好FF相关。
