PDF(Pubmed)
Abstract：
Surveillance data for Ancylostoma spp. and the A. caninum benzimidazole treatment resistance associated F167Y polymorphism using molecular diagnostics was obtained in a large population of dogs from the United States and Canada. Real-time PCR (qPCR) for Ancylostoma spp. and allele-specific qPCR detecting a single nucleotide polymorphism (SNP) F167Y was used in 262,872 canine stool samples collected between March and December of 2022. Ancylostoma spp. was found at an overall prevalence of 2.5% (6538/262,872), with the highest prevalence in the Southern US, 4.4% (4490/103,095), and the lowest prevalence in Canada 0.6% (101/15,829). The A. caninum F167Y polymorphism was found with the highest prevalence (13.4%, n = 46/343) in the Western US and the lowest in Canada at 4.1% (4/97). The F167Y polymorphism was detected every month over the 10-month collection period. Seasonal distribution showed a peak in June for both Ancylostoma spp. (3.08%, 547/17,775) and A. caninum F167Y (12.25%, 67/547). However, the A. caninum F167Y polymorphism prevalence was highest in September (13.9%, 119/856). Age analysis indicates a higher prevalence of both hookworm infections and occurrence of resistant isolates in puppies. The breeds with the highest F167Y polymorphism prevalence in Ancylostoma spp. detected samples were poodles (28.9%), followed by Bernese Mountain dogs (25%), Cocker spaniels (23.1%), and greyhounds (22.4%). Our data set describes widespread geographic distribution of the A. caninum benzimidazole resistance associated F167Y polymorphism in the United States and Canada, with no clear seasonality compared to the Ancylostoma spp. prevalence patterns. The F167 polymorphism was present in all geographic areas with detected hookworms, including Canada. Our study highlights that the F167Y polymorphism is represented in many dog breeds, including greyhounds.
摘要：
Ancylostomaspp的监测数据。并且使用分子诊断在来自美国和加拿大的大量狗中获得了与犬苯并咪唑治疗抗性相关的F167Y多态性。Ancylostomaspp的实时PCR（qPCR）。和等位基因特异性qPCR检测单核苷酸多态性（SNP）F167Y在2022年3月至12月之间收集的262,872犬类粪便样本中使用。环形藻属。总体患病率为2.5%(6538/262,872)，在美国南部患病率最高，4.4%（4490/103,095），加拿大患病率最低，为0.6%(101/15,829)。A.caninumF167Y多态性的患病率最高(13.4%,n=46/343），美国西部最低，加拿大为4.1%（4/97）。在10个月的收集期内每个月检测F167Y多态性。两个Ancylostomaspp的季节性分布均在6月达到峰值。（3.08%，547/17,775）和A.caninumF167Y（12.25%，67/547)。然而,9月A.caninumF167Y多态性患病率最高(13.9%，119/856）。年龄分析表明，幼犬中钩虫感染和耐药分离株的患病率更高。在Ancylostomaspp中F167Y多态性患病率最高的品种。检测到的样本是贵宾犬(28.9%)，其次是伯尔尼山的狗（25%），可卡犬（23.1％），和灰狗(22.4%)。我们的数据集描述了美国和加拿大的犬苯并咪唑抗性相关F167Y多态性的广泛地理分布，与Ancylostomaspp相比，没有明显的季节性。流行模式。F167多态性存在于所有检测到钩虫的地理区域，包括加拿大。我们的研究强调，F167Y多态性在许多犬种中都有代表，包括灰狗.
