Abstract：
OBJECTIVE: Radiotherapy showed the potential to effectively kill the cysts of pulmonary cystic echinococcosis (CE). However, little is known about its safety. This study was designed to investigate the safety of three-dimensional conformal radiotherapy (3D-CRT) on the normal lung tissue adjacent to the cyst and blood of sheep naturally infected with pulmonary CE.
METHODS: Twenty pulmonary CE sheep were randomly divided into control group (n = 5) and radiation groups with a dose of 30 Gray (Gy) (n = 5), 45 Gy (n = 5), and 60 Gy (n = 5), respectively. Animals in control group received no radiation. Heat shock protein 70 (Hsp70), tumor growth factor-β (TGF-β), matrix metalloproteinase-2 (MMP-2) and MMP-9 in the lung tissues adjacent to the cysts, which were considered to be closely related to the pathogenesis of CE, were evaluated after 3D-CRT. A routine blood test was conducted.
RESULTS: The results showed that there were multiple cysts of various sizes with protoscoleces in the lung tissues of sheep, and necrotic cysts were found after 3D-CRT. 3D-CRT significantly increased the mRNA level of Hsp70, enhanced the protein level of TGF-β and slightly increased the expression of MMP-2 and MMP-9 in lung tissues adjacent to the cysts. 3D-CRT did not significantly alter the amount of WBC, HB and PLT in sheep blood.
CONCLUSIONS: The results suggested that 3D-CRT may suppress the inflammation and induce less damage of the normal lung tissues and blood. We preliminarily showed that 3D-CRT under a safe dose may be used to treat pulmonary CE.
METHODS: Twenty pulmonary CE sheep were randomly divided into control group (n = 5) and radiation groups with a dose of 30 Gray (Gy) (n = 5), 45 Gy (n = 5), and 60 Gy (n = 5), respectively. Animals in control group received no radiation. Heat shock protein 70 (Hsp70), tumor growth factor-β (TGF-β), matrix metalloproteinase-2 (MMP-2) and MMP-9 in the lung tissues adjacent to the cysts, which were considered to be closely related to the pathogenesis of CE, were evaluated after 3D-CRT. A routine blood test was conducted.
RESULTS: The results showed that there were multiple cysts of various sizes with protoscoleces in the lung tissues of sheep, and necrotic cysts were found after 3D-CRT. 3D-CRT significantly increased the mRNA level of Hsp70, enhanced the protein level of TGF-β and slightly increased the expression of MMP-2 and MMP-9 in lung tissues adjacent to the cysts. 3D-CRT did not significantly alter the amount of WBC, HB and PLT in sheep blood.
CONCLUSIONS: The results suggested that 3D-CRT may suppress the inflammation and induce less damage of the normal lung tissues and blood. We preliminarily showed that 3D-CRT under a safe dose may be used to treat pulmonary CE.
摘要：
目的：放射治疗显示出有效杀死肺囊性包虫病（CE）囊肿的潜力。然而,人们对它的安全性知之甚少。本研究旨在探讨三维适形放疗（3D-CRT）对自然感染肺CE的绵羊囊肿和血液附近的正常肺组织的安全性。
方法：将20只肺CE绵羊随机分为对照组（n=5）和剂量为30格雷（Gy）的辐射组（n=5）。45Gy(n=5),和60Gy（n=5），分别。对照组动物不接受辐射。热休克蛋白70(Hsp70),肿瘤生长因子-β（TGF-β），囊肿附近肺组织中的基质金属蛋白酶-2（MMP-2）和MMP-9，被认为与CE的发病机制密切相关，在3D-CRT后进行评估。进行了血常规检查。
结果：结果表明，绵羊的肺组织中存在多个大小各异的囊肿，3D-CRT后发现坏死囊肿。3D-CRT可显著提高囊肿旁肺组织中Hsp70的mRNA水平，增强TGF-β的蛋白水平，并略微增加MMP-2和MMP-9的表达。3D-CRT并没有显著改变白细胞的数量，绵羊血液中的HB和PLT。
结论：结果表明，3D-CRT可以抑制炎症反应，减轻正常肺组织和血液的损伤。我们初步表明，在安全剂量下3D-CRT可用于治疗肺部CE。
方法：将20只肺CE绵羊随机分为对照组（n=5）和剂量为30格雷（Gy）的辐射组（n=5）。45Gy(n=5),和60Gy（n=5），分别。对照组动物不接受辐射。热休克蛋白70(Hsp70),肿瘤生长因子-β（TGF-β），囊肿附近肺组织中的基质金属蛋白酶-2（MMP-2）和MMP-9，被认为与CE的发病机制密切相关，在3D-CRT后进行评估。进行了血常规检查。
结果：结果表明，绵羊的肺组织中存在多个大小各异的囊肿，3D-CRT后发现坏死囊肿。3D-CRT可显著提高囊肿旁肺组织中Hsp70的mRNA水平，增强TGF-β的蛋白水平，并略微增加MMP-2和MMP-9的表达。3D-CRT并没有显著改变白细胞的数量，绵羊血液中的HB和PLT。
结论：结果表明，3D-CRT可以抑制炎症反应，减轻正常肺组织和血液的损伤。我们初步表明，在安全剂量下3D-CRT可用于治疗肺部CE。
