Abstract：
Fructans have long been known with their role in protecting organisms against various stress factors due to their ability to induce controlled dehydration and support membrane stability. Considering the vital importance of such features in cryo-technologies, this study aimed to explore the cryoprotective efficacy of fructans in mammalian cell systems where structurally different fructan polymers were examined on in vitro cell models derived from organs such as the liver, frequently used in transplantation, osteoblast, and cord cells, commonly employed in cell banking, as well as human seminal fluids that are of vital importance in assisted reproductive technology. To gain insights into the fructan/membrane interplay, structural differences were linked to rheological properties as well as to lipid membrane interactions where both fluorescein leakage from unilamellar liposomes and membrane integrity of osteoblast cells were monitored. High survival rates obtained with human endothelial, osteoblast and liver cells for up to two months clearly showed that fructans could be considered as effective non-permeating cryoprotectants, especially for extended periods of cryopreservation. In trials with human seminal fluid, short chained levan in combination with human serum albumin and glycerol proved very effective in preserving semen samples across multiple patients without any morphological abnormalities.
摘要：
由于果聚糖诱导受控脱水和支持膜稳定性的能力，它们在保护生物体免受各种应激因素的作用早已为人所知。考虑到这些功能在冷冻技术中的重要性，这项研究旨在探索在哺乳动物细胞系统中果聚糖的冷冻保护功效，其中结构不同的果聚糖聚合物在体外细胞模型上进行了检查，从器官如肝脏，经常用于移植，成骨细胞,和脐带细胞，常用于细胞库，以及在辅助生殖技术中至关重要的人类精液。为了深入了解果聚糖/膜的相互作用，结构差异与流变学特性以及脂质膜相互作用有关，其中监测单层脂质体的荧光素渗漏和成骨细胞的膜完整性。人内皮获得的高存活率，成骨细胞和肝细胞长达两个月清楚地表明，果聚糖可以被认为是有效的非渗透性冷冻保护剂，特别是长时间的冷冻保存。在人体精液试验中，短链果聚糖与人血清白蛋白和甘油的组合被证明可以非常有效地保存多名患者的精液样本，而没有任何形态学异常。
