Abstract：
Maternal diabetes during pregnancy can affect the neurological development of offspring. Glial cell-derived neurotrophic factor (GDNF), neurturin (NRTN), and neural cell adhesion molecules (NCAM) are three important proteins for brain development. Therefore, this study aimed to investigate the impacts of the mentioned neurotrophic factors in the hippocampal dentate gyrus (DG) of rat offspring born to diabetic mothers.
Wistar female rats were randomly allocated into diabetic (STZ-D) [(45 mg/kg BW, STZ (Streptozotocin), i.p)], diabetic + NPH insulin (STZ-INS) [(4-6 unit/kg/day SC)], and control groups. The animals in all groups were mated by non-diabetic male rats. Two weeks after birth, male pups from each group were sacrificed and then protein contents of GDNF, NRTN, and NCAM were evaluated using immunohistochemistry.
The study found that the expression of GDNF and NRTN in the hippocampus of diabetic rat offspring was significantly higher compared to the diabetic+ insulin and control groups, respectively (P < 0.01, P < 0.001). Additionally, the expression of NCAM was significantly higher in the diabetic group the diabetic+ insulin and control groups (P < 0.01, P < 0.001).
The results of the study revealed that diabetes during pregnancy significantly impacts the distribution pattern of GDNF, NRTN, and NCAM in the hippocampus of rat neonates.
Wistar female rats were randomly allocated into diabetic (STZ-D) [(45 mg/kg BW, STZ (Streptozotocin), i.p)], diabetic + NPH insulin (STZ-INS) [(4-6 unit/kg/day SC)], and control groups. The animals in all groups were mated by non-diabetic male rats. Two weeks after birth, male pups from each group were sacrificed and then protein contents of GDNF, NRTN, and NCAM were evaluated using immunohistochemistry.
The study found that the expression of GDNF and NRTN in the hippocampus of diabetic rat offspring was significantly higher compared to the diabetic+ insulin and control groups, respectively (P < 0.01, P < 0.001). Additionally, the expression of NCAM was significantly higher in the diabetic group the diabetic+ insulin and control groups (P < 0.01, P < 0.001).
The results of the study revealed that diabetes during pregnancy significantly impacts the distribution pattern of GDNF, NRTN, and NCAM in the hippocampus of rat neonates.
摘要：
背景：母亲妊娠期糖尿病可影响后代的神经发育。胶质细胞源性神经营养因子(GDNF),Neurturin（NRTN），神经细胞粘附分子(NCAM)是大脑发育的三种重要蛋白质。因此,这项研究旨在探讨上述神经营养因子对糖尿病母亲所生的大鼠后代海马齿状回（DG）的影响。
方法：Wistar雌性大鼠随机分为糖尿病（STZ-D）[（45mg/kgBW，STZ（链脲佐菌素），i.p)],糖尿病+NPH胰岛素（STZ-INS）[（4-6单位/kg/天SC）]，和对照组。所有组中的动物均由非糖尿病雄性大鼠交配。出生两周后，处死各组雄性幼崽，然后处死GDNF的蛋白质含量，NRTN,和NCAM使用免疫组织化学进行评估。
结果：研究发现，糖尿病大鼠子代海马GDNF和NRTN的表达明显高于糖尿病+胰岛素组和对照组，分别为(P<0.01,P<0.001)。此外,NCAM的表达在糖尿病组，糖尿病胰岛素组和对照组中明显升高（P<0.01，P<0.001）。
结论：研究结果表明，妊娠期糖尿病显著影响GDNF的分布，NRTN,和大鼠新生儿海马中的NCAM。
方法：Wistar雌性大鼠随机分为糖尿病（STZ-D）[（45mg/kgBW，STZ（链脲佐菌素），i.p)],糖尿病+NPH胰岛素（STZ-INS）[（4-6单位/kg/天SC）]，和对照组。所有组中的动物均由非糖尿病雄性大鼠交配。出生两周后，处死各组雄性幼崽，然后处死GDNF的蛋白质含量，NRTN,和NCAM使用免疫组织化学进行评估。
结果：研究发现，糖尿病大鼠子代海马GDNF和NRTN的表达明显高于糖尿病+胰岛素组和对照组，分别为(P<0.01,P<0.001)。此外,NCAM的表达在糖尿病组，糖尿病胰岛素组和对照组中明显升高（P<0.01，P<0.001）。
结论：研究结果表明，妊娠期糖尿病显著影响GDNF的分布，NRTN,和大鼠新生儿海马中的NCAM。
