Abstract：
Regulatory RNAs in bacteria are known to act by base pairing with other RNAs. Interactions between two partner RNAs can be investigated by electrophoretic mobility shift assays. The regions predicted to be engaged in base pairing are analyzed by introducing mutations in one RNA that prevent RNA-RNA complex formation. Next, base pairing is restored by introducing complementary mutations in its partner RNA. Here, we describe the mutational strategy and experimental methods used to validate specific base pairing between two RNA species.
摘要：
已知细菌中的调控RNA通过与其他RNA的碱基配对起作用。两个配偶体RNA之间的相互作用可以通过电泳迁移率变化测定来研究。通过在一个RNA中引入防止RNA-RNA复合物形成的突变来分析预测参与碱基配对的区域。接下来,通过在其伴侣RNA中引入互补突变来恢复碱基配对。这里,我们描述了用于验证两个RNA物种之间特定碱基配对的突变策略和实验方法。
