Abstract：
Effects of butyric acid, a bacterial metabolite implicated in periodontitis progression, have never been examined on oral melanocytes. Herein, primary human epidermal melanocytes were used as a model for oral melanocytes. Results show the adverse effects of butyric acid (sodium butyrate; NaB) on them, which comprise marked cytotoxicity at higher concentrations (>1 mM) and robust differentiation at lower nontoxic concentrations. NaB did not alter MITF protein levels; however, it stimulated tyrosinase protein synthesis and inhibited tyrosinase activity, with no changes in cellular melanin. NaB did not affect oxidative stress, although it induced significant levels of the pro-inflammatory cytokine IL-6.
摘要：
丁酸的影响，一种与牙周炎进展有关的细菌代谢产物，从未在口腔黑素细胞上检查过。在这里，原代人表皮黑素细胞被用作口腔黑素细胞的模型。结果显示丁酸(丁酸钠;NaB)对它们的不利影响,其包括在较高浓度(>1mM)下显著的细胞毒性和在较低无毒浓度下的强分化。NaB不会改变MITF蛋白水平；然而，它刺激酪氨酸酶蛋白质合成并抑制酪氨酸酶活性，细胞黑色素没有变化。NaB不影响氧化应激，尽管它诱导了显着水平的促炎细胞因子IL-6。
