Abstract：
BACKGROUND: Acinetobacter baumannii is an opportunistic hospital pathogen with high antibiotic resistance, and the ability to produce biofilm. This study aimed to investigate epsA, ompA, and bap genes involved in biofilm formation in MDR and XDR clinical isolates of Acinetobacter baumannii in Khorramabad, Iran.
METHODS: In this study, 79 A. baumannii isolates were collected from various samples of the patients admitted to tertiary hospitals in Khorramabad city, Iran, between January and August 2019. After performing the semi-quantitative evaluation of biofilm production by microtiter plate assay, screening of isolates carrying epsA, ompA, and bap genes was done by PCR method. Finally, statistical analyses were conducted using SPSS 22.
RESULTS: Among 79 A. baumannii isolates, 52% XDR, 40% MDR, and 16% non-XDRMDR isolates were found to be biofilm producers. All XDR and 94% MDR isolates had ompA and epsA genes, and bap genes were detected among > 80% of these isolates. Moreover, the presence of biofilm-related genes and biofilm production among non-XDRMDR isolates were less than among resistant isolates (p≤ 0.01).
CONCLUSIONS: Based on the results, biofilm production and simultaneous presence of epsA, ompA, and bap genes among MDR, and XDR A. baumannii isolates have been found to be significantly more than non-XDR-MDR isolates.
METHODS: In this study, 79 A. baumannii isolates were collected from various samples of the patients admitted to tertiary hospitals in Khorramabad city, Iran, between January and August 2019. After performing the semi-quantitative evaluation of biofilm production by microtiter plate assay, screening of isolates carrying epsA, ompA, and bap genes was done by PCR method. Finally, statistical analyses were conducted using SPSS 22.
RESULTS: Among 79 A. baumannii isolates, 52% XDR, 40% MDR, and 16% non-XDRMDR isolates were found to be biofilm producers. All XDR and 94% MDR isolates had ompA and epsA genes, and bap genes were detected among > 80% of these isolates. Moreover, the presence of biofilm-related genes and biofilm production among non-XDRMDR isolates were less than among resistant isolates (p≤ 0.01).
CONCLUSIONS: Based on the results, biofilm production and simultaneous presence of epsA, ompA, and bap genes among MDR, and XDR A. baumannii isolates have been found to be significantly more than non-XDR-MDR isolates.
摘要：
背景：鲍曼不动杆菌是一种具有高抗生素耐药性的机会性医院病原菌，以及产生生物膜的能力。本研究旨在调查epsA，ompa,在霍拉马巴德鲍曼不动杆菌的MDR和XDR临床分离株中参与生物膜形成的bap基因，伊朗。
方法：在本研究中，从霍拉马巴德市三级医院收治的患者的各种样本中收集了79株鲍曼不动杆菌分离株，伊朗,2019年1月至8月。通过微量滴定板测定法对生物膜的产生进行半定量评估后，筛选携带epsA的分离株，ompa,用PCR方法检测bap基因。最后,使用SPSS22进行统计分析。
结果：在79株鲍曼不动杆菌中，52%XDR,40%MDR,发现16%的非XDRMDR分离株是生物膜生产者。所有XDR和94%MDR分离株都有ompA和epsA基因，在80%以上的分离株中检测到bap基因。此外，非XDRMDR分离株中生物膜相关基因的存在和生物膜产量低于耐药分离株(p≤0.01).
结论：根据结果，生物膜的产生和同时存在的epsA，ompa,MDR中的bap基因，已发现XDR鲍曼不动杆菌分离株显著高于非XDR-MDR分离株。
方法：在本研究中，从霍拉马巴德市三级医院收治的患者的各种样本中收集了79株鲍曼不动杆菌分离株，伊朗,2019年1月至8月。通过微量滴定板测定法对生物膜的产生进行半定量评估后，筛选携带epsA的分离株，ompa,用PCR方法检测bap基因。最后,使用SPSS22进行统计分析。
结果：在79株鲍曼不动杆菌中，52%XDR,40%MDR,发现16%的非XDRMDR分离株是生物膜生产者。所有XDR和94%MDR分离株都有ompA和epsA基因，在80%以上的分离株中检测到bap基因。此外，非XDRMDR分离株中生物膜相关基因的存在和生物膜产量低于耐药分离株(p≤0.01).
结论：根据结果，生物膜的产生和同时存在的epsA，ompa,MDR中的bap基因，已发现XDR鲍曼不动杆菌分离株显著高于非XDR-MDR分离株。
