PDF(Pubmed)
Abstract：
Oxidative stress in the skin, induced by an unhealthy lifestyle and exposure to UVB radiation, leads to skin aging, including reduced elasticity, formation of wrinkles, moisture loss, and inflammation. In a previous study, we revealed the photoaging effects of enzyme-treated caviar extract (CV) by regulating collagen and hyaluronic acid synthase, melanogenesis, anti-oxidant mechanisms, and inflammation in a UVB irradiation-induced mice model. HPLC and MALDI-TOF were performed to determine the effect of enzyme treatment on the free amino acid contents and peptide molecular weight in supercritical caviar extract. As results of the analysis, CV is mainly composed of low-molecular-weight peptides consisting of leucine, tyrosine, and phenylalanine. Based on our in vitro and in vivo study, we conducted a clinical trial to assess the skin anti-aging efficacy of CV. In this randomized, double-blind, placebo-controlled trial, we measured indicators related to elasticity, wrinkles, and skin hydration at 4 and 8 weeks after consumption of CV. The subjects were categorized into caviar, combination, and placebo groups. After 4 weeks, skin hydration, dermal hydration, and transepidermal water loss all showed significant improvement. Furthermore, after 8 weeks, skin elasticity indexes-R2 (total elasticity), R5 (net elasticity), and R7 (ratio of elastic recovery to total deformation)-exhibited significant increases. Improvement in wrinkle indicators (Rmax, Ra, and Rz) and the whitening indicator melanin pigment was also observed. This is the first report showing that CV has significant skin anti-aging efficacy on human skin. In conclusion, our study suggests that CV can be used as skin anti-aging nutraceuticals through positive effects on skin condition in clinical trials.
摘要：
皮肤中的氧化应激，由不健康的生活方式和暴露于UVB辐射引起的，导致皮肤老化，包括弹性降低，皱纹的形成，水分损失,和炎症。在之前的研究中,我们揭示了酶处理的鱼子酱提取物（CV）通过调节胶原蛋白和透明质酸合酶的光老化效应，黑色素生成，抗氧化机制，和UVB辐射诱导的小鼠模型中的炎症。进行HPLC和MALDI-TOF以确定酶处理对超临界鱼子酱提取物中游离氨基酸含量和肽分子量的影响。作为分析的结果，CV主要由亮氨酸组成的低分子量肽组成，酪氨酸,和苯丙氨酸.根据我们的体外和体内研究，我们进行了一项临床试验,以评估CV的皮肤抗衰老功效.在这个随机的，双盲,安慰剂对照试验,我们测量了与弹性相关的指标，皱纹,消耗CV后4周和8周的皮肤水合作用。受试者被分类为鱼子酱，组合,和安慰剂组。4周后,皮肤水合作用,皮肤水合作用,经表皮失水均有明显改善。此外，8周后,皮肤弹性指数-R2(总弹性)，R5（净弹性），和R7(弹性恢复与总变形的比率)表现出显著的增加。皱纹指标的改善(Rmax，Ra,和Rz），还观察到美白指示剂黑色素。这是第一份报告显示CV对人类皮肤具有显著的皮肤抗衰老功效。总之,我们的研究表明,在临床试验中,CV通过对皮肤状况产生积极作用,可作为皮肤抗衰老的营养品.
