PDF(Pubmed)
Abstract：
Plant leaves, such as those from Mangifera indica, represent a potential utilization of waste due to their richness in bioactive compounds. Supercritical CO2 allows these compounds to be incorporated into various matrices by impregnation. Combined with its ability to generate polymeric scaffolds, it represents an attractive strategy for the production of biomedical devices. For this purpose, conjugated polymeric scaffolds of biodegradable PLGA (poly(lactic-co-glycolic acid)) and PEDOT:PSS (poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate)), generated in situ by foaming, were employed for the supercritical impregnation of ethanolic mango leaves extract (MLE) in tissue engineering as a potential application. The extraction of MLE was performed by Enhanced Solvent Extraction. The effects of pressure (120-300 bar), temperature (35-55 °C), and depressurization rate (1-50 bar/min) on the physical/conductive properties and the impregnation of MLE were studied. The scaffolds have been characterized by liquid displacement, scanning electron microscope, resistance to conductivity techniques, measurements of impregnated load, antioxidant capacity and antimicrobial activity. Porosity values ranging 9-46% and conductivity values between 10-4-10-5 S/cm were obtained. High pressures, low temperatures and rapid depressurization favored the impregnation of bioactive compounds. Scaffolds with remarkable antioxidant activity were obtained (75.2-87.3% oxidation inhibition), demonstrating the ability to inhibit S. aureus bacterial growth (60.1 to 71.4%).
摘要：
植物叶子，比如来自Mangiferaindica的，由于其丰富的生物活性化合物，代表了废物的潜在利用。超临界CO2允许这些化合物通过浸渍结合到各种基质中。结合其产生聚合物支架的能力，它代表了生物医学设备生产的一个有吸引力的策略。为此,可生物降解的PLGA（聚（乳酸-乙醇酸））和PEDOT：PSS（聚（3,4-亚乙基二氧噻吩）-聚（苯乙烯磺酸盐））的共轭聚合物支架，通过发泡原位产生，作为组织工程中的潜在应用，将其用于乙醇芒果叶提取物（MLE）的超临界浸渍。MLE的提取通过增强溶剂萃取进行。压力的影响（120-300巴），温度（35-55°C），研究了减压速率（1-50bar/min）对MLE的物理/导电性能和浸渍性能的影响。支架的特点是液体置换，扫描电子显微镜，抗导电性技术，浸渍载荷的测量，抗氧化能力和抗菌活性。获得9-46%的孔隙率值和10-4-10-5S/cm之间的电导率值。高压,低温和快速减压有利于生物活性化合物的浸渍。获得了具有显着抗氧化活性的支架（氧化抑制75.2-87.3％），证明抑制金黄色葡萄球菌细菌生长的能力（60.1至71.4％）。
