Abstract：
Molecular surveillance of canine tick-borne pathogens (TBPs) in Bangladesh has constantly been undervalued. Therefore, the emergence of new pathogens often remains undetected. This study aimed to screen tick-borne pathogens in stray dogs and ticks in the Dhaka metropolitan area (DMA). Eighty-five dog blood and 53 ticks were collected in six city districts of DMA from September 2022 to January 2023. The ticks were identified by morphology. Screening of TBPs was performed by polymerase chain reaction (PCR), followed by sequencing. The PCR assays were conducted to analyze the 18S rRNA (Babesia gibsoni, B. vogeli, and Hepatozoon canis), 16S rRNA (Anaplasma phagocytophilum, A. platys, and A. bovis), gltA (Ehrlichia canis and Rickettsia spp.), flagellin B (Borrelia spp.) and 16-23S rRNA (Bartonella spp.). Three tick species, Rhipicephalus sanguineus (50/53), R. microplus (1/53), and Haemaphysalis bispinosa (2/53), were identified. Babesia gibsoni (38 out of 85) and A. platys (7 out of 85) were detected in dog blood. In contrast, four pathogens, B. gibsoni (1 out of 53), B. vogeli (1 out of 53), H. canis (22 out of 53), and A. platys (1 out of 53), were detected in the ticks. However, the detection rates of TBPs in dog blood and ticks were not correlated in this study. The phylogenetic analyses suggested that a single genotype for each of the four pathogens is circulating in DMA. This study reports the existence of B. vogeli, H. canis, and A. platys in Bangladesh for the first time.
摘要：
孟加拉国犬蜱传病原体（TBP）的分子监测一直被低估。因此,新病原体的出现往往未被发现。这项研究旨在筛选达卡都会区（DMA）的流浪狗和蜱中的蜱传播病原体。从2022年9月至2023年1月，在DMA的六个城市地区收集了85个狗血和53个蜱。通过形态学鉴定蜱。通过聚合酶链反应（PCR）进行TBP的筛选，其次是测序。进行PCR测定以分析18SrRNA(Babesiagibsoni，B.Vogeli,和肝虫犬)，16SrRNA（吞噬细胞无性体，A.Platys,和A.bovis)，gltA（犬埃里希氏菌和立克次体属。),鞭毛蛋白B（疏螺旋体属。）和16-23SrRNA（Bartonellaspp。).三个蜱物种，血根虫(50/53)，R.microplus(1/53)，和双品红血丝(2/53)，已确定。在狗血液中检测到gibsoni巴贝斯虫（85个中的38个）和A.platys（85个中的7个）。相比之下,四种病原体，B.gibsoni(53人中有1人)，B.vogeli（53人中有1人），H.canis（53个中的22个），和A.platys（53个中的1个），在蜱中检测到。然而,在这项研究中，狗血和蜱中TBP的检出率没有相关性。系统发育分析表明，四种病原体中每种病原体的单个基因型在DMA中循环。这项研究报道了B.vogeli的存在，H.canis,和A.Platys第一次在孟加拉国。
