Abstract：
Chitin, the second most abundant biomolecule after cellulose in nature, is a significant aquaculture by-product, and is estimated at 6-8 million tons annually. Chitin is composed of monomeric N-acetylglucosamine (NAG) which can be seen as an alternative feedstock for biotechnology. Microbial functional lipids have gained attention due to their bioactivity and sustainable production. In this study, a new oleaginous yeast strain named Sakaguchia sp. HKC2 was found to be able to use NAG as the carbon source for growth and accumulate functional lipids such as PUFAs and carotenoids. When cultured on the NAG-containing medium, strain HKC2 exhibited slower growth and slower intracellular lipid accumulation compared to those on a glucose-containing medium. However, the lipids obtained from HKC2 grown on NAG medium were richer in PUFAs. Notably, torularhodin-a powerful bioactive carotenoid-was found in all HKC2 cultures on NAG, while torulene was abundant in glucose medium. These findings highlight a novel avenue for utilizing aquatic by-products and unlocking their potential.
摘要：
甲壳素,自然界中仅次于纤维素的第二丰富的生物分子，是一种重要的水产养殖副产品，估计每年有600万至800万吨。几丁质由单体N-乙酰葡糖胺(NAG)组成,其可以被视为生物技术的替代原料。微生物功能性脂质由于其生物活性和可持续生产而受到关注。在这项研究中,一种新的产油酵母菌株，名为Sakaguchiasp.发现HKC2能够使用NAG作为碳源用于生长并积累功能性脂质如PUFA和类胡萝卜素。当在含NAG的培养基上培养时，与含葡萄糖的培养基相比，HKC2菌株表现出较慢的生长和较慢的细胞内脂质积累。然而,从NAG培养基上生长的HKC2获得的脂质富含PUFA。值得注意的是,在NAG的所有HKC2培养物中都发现了Torularhodin-一种强大的生物活性类胡萝卜素，而torulene在葡萄糖培养基中含量丰富。这些发现凸显了利用水生副产品并释放其潜力的新途径。
