Abstract：
Antibiotic use in aquaculture for increase yield has been established over time; however, consumption of such fish may lead to inadvertent intake of sub therapeutic doses of antibiotics. Therefore, this study aimed to investigate the extent of antibiotic use in fish farming and ciprofloxacin and gentamicin residues in fish that are consumed in Lagos, Nigeria. It was conducted in two parts: a survey of 60 fish producers to assess their use of antibiotics and an analysis of ciprofloxacin and gentamicin residues in fish organs using high-performance liquid chromatography (HPLC). The survey found that twenty-nine (48%) of the farms used antibiotics, with oxytetracycline being the most common (20%), followed by ciprofloxacin (15%); gentamicin (5%); neomycin (8.33%); and probiotics (13%). HPLC analysis of fish liver and fillet samples showed that Lagos Island had the lowest residual ciprofloxacin dose (0.3014ug/g), while Lagos mainland had the greatest residual ciprofloxacin dosage (113.78765ug/g). The mean gentamicin residue in flesh of fish from farms in Lagos Island LGA was 0.37ug/g while that obtained for liver of fish samples from Surulere LGA was 2.12ug/g). The largest (5.3240ug/g) and lowest (0.2661ug/g) residual gentamicin antibiotic concentrations were found in fishes harvested from farms located in Surulere and Lagos Island, respectively. The residue levels were within the permitted range set by the WHO, but continuous regulatory surveillance is necessary to prevent antimicrobial resistance spread and improved food safety. The study has revealed that fish produced in the study area contained residues of the commonly used antibiotics hence farmers should be encouraged to consult veterinarians in cases of disease treatment for judicious use of antibiotics while self-medication and purchase of antibiotics from vendors should be discouraged.
摘要：
随着时间的推移，在水产养殖中使用抗生素提高产量已经确立；然而，食用这种鱼可能会导致无意中摄入亚治疗剂量的抗生素。因此,这项研究旨在调查在鱼类养殖中使用抗生素的程度以及在拉各斯食用的鱼类中的环丙沙星和庆大霉素残留，尼日利亚。分两个部分进行：对60个鱼类生产者进行调查，以评估他们对抗生素的使用情况，以及使用高效液相色谱（HPLC）分析鱼类器官中环丙沙星和庆大霉素的残留。调查发现29个(48%)农场使用抗生素,土霉素是最常见的（20％），其次是环丙沙星（15％）;庆大霉素（5％）;新霉素（8.33％）;和益生菌（13％）。鱼肝和鱼片样品的HPLC分析表明，拉各斯岛的环丙沙星残留剂量最低（0.314ug/g），而拉各斯大陆的环丙沙星残留量最大(113.78765ug/g)。拉各斯岛LGA农场鱼肉中庆大霉素的平均残留量为0.37ug/g，而从SurulereLGA中获得的鱼肉样品的平均庆大霉素残留量为2.12ug/g）。最大（5.340ug/g）和最低（0.2661ug/g）残留庆大霉素抗生素浓度是在苏鲁莱尔和拉各斯岛农场收获的鱼类中发现的，分别。残留水平在世界卫生组织设定的允许范围内，但是持续的监管监督对于防止抗菌素耐药性传播和改善食品安全是必要的。该研究表明，在研究区域生产的鱼中含有常用抗生素的残留物，因此应鼓励农民在疾病治疗的情况下咨询兽医，以明智地使用抗生素，同时不鼓励自我用药和从供应商处购买抗生素。
