PDF(Pubmed)
Abstract：
The CRISPR system is a revolutionary genome editing tool that has the potential to revolutionize the field of cancer research and therapy. The ability to precisely target and edit specific genetic mutations that drive the growth and spread of tumors has opened up new possibilities for the development of more effective and personalized cancer treatments. In this review, we will discuss the different CRISPR-based strategies that have been proposed for cancer therapy, including inactivating genes that drive tumor growth, enhancing the immune response to cancer cells, repairing genetic mutations that cause cancer, and delivering cancer-killing molecules directly to tumor cells. We will also summarize the current state of preclinical studies and clinical trials of CRISPR-based cancer therapy, highlighting the most promising results and the challenges that still need to be overcome. Safety and delivery are also important challenges for CRISPR-based cancer therapy to become a viable clinical option. We will discuss the challenges and limitations that need to be overcome, such as off-target effects, safety, and delivery to the tumor site. Finally, we will provide an overview of the current challenges and opportunities in the field of CRISPR-based cancer therapy and discuss future directions for research and development. The CRISPR system has the potential to change the landscape of cancer research, and this review aims to provide an overview of the current state of the field and the challenges that need to be overcome to realize this potential.
摘要：
CRISPR系统是一种革命性的基因组编辑工具，有可能彻底改变癌症研究和治疗领域。精确靶向和编辑驱动肿瘤生长和扩散的特定基因突变的能力为开发更有效和个性化的癌症治疗开辟了新的可能性。在这次审查中,我们将讨论针对癌症治疗提出的基于CRISPR的不同策略，包括驱动肿瘤生长的失活基因，增强对癌细胞的免疫反应，修复导致癌症的基因突变，并将抗癌分子直接传递给肿瘤细胞。我们还将总结基于CRISPR的癌症治疗的临床前研究和临床试验的现状，强调最有希望的结果和仍然需要克服的挑战。安全性和递送也是基于CRISPR的癌症治疗成为可行的临床选择的重要挑战。我们将讨论需要克服的挑战和限制，比如脱靶效应，安全,并运送到肿瘤部位。最后,我们将概述基于CRISPR的癌症治疗领域当前的挑战和机遇，并讨论未来的研究和发展方向。CRISPR系统有可能改变癌症研究的格局，本次审查旨在概述该领域的现状以及实现这一潜力所需克服的挑战。
