关键词: Confocal laser scanning microscopy Easydo activator Irrigation Root canal therapy Scanning electron microscopy Sealer penetration Smear layer removal

Mesh : Humans Dental Care Gutta-Percha Microscopy, Confocal Smear Layer Ultrasonics

来  源:   DOI:10.1186/s12903-023-03833-y   PDF(Pubmed)

Abstract:
The effects of Easydo Activator (EA), a new sonic irrigation system, on sealer penetration at the root apex were compared to needle irrigation (NI) and passive ultrasonic irrigation (PUI) in this study. Forty-two single-rooted teeth were prepared and randomly divided into three groups (n = 14): group 1: NI; group 2: PUI; and group 3: EA. A solution of 3% sodium hypochlorite (NaOCl) was used for irrigation. Nine teeth in each group were filled with AH Plus sealer mixed with CY5 fluorescent dye and a single gutta-percha cone. The sealer penetration area, maximum penetration depth and percentage of sealer penetration at 5 mm and 1 mm from the apex were analyzed by confocal laser scanning microscopy (CLSM). The remaining 5 teeth in each group were subjected to test smear layer scores by scanning electron microscopy (SEM). The CLSM evaluation showed that increases in the area, depth and percentage of sealer penetration were detected at 1 and 5 mm from the root apex in the PUI group compared with the NI group, and greater increases were observed in the EA group (P < 0.05). The SEM experiment showed that the lowest scores for the smear layer and debris removal were achieved by the EA group when compared with the PUI and NI groups (P < 0.05). In conclusion, EA was superior to PUI and NI regarding sealer penetration at the root apex during endodontic treatment, and it could provide a new technical idea for clinical root canal therapy.
摘要:
Easydo激活剂(EA)的效果,一种新的声波灌溉系统,在这项研究中,将根尖处的密封剂渗透与针头冲洗(NI)和被动超声冲洗(PUI)进行了比较。制备42颗单根牙齿,随机分为三组(n=14):第1组:NI;第2组:PUI;第3组:EA。使用3%次氯酸钠(NaOCl)溶液进行灌溉。每组九颗牙齿填充AHPlus密封剂,并混合CY5荧光染料和单个古塔胶锥。密封剂渗透区域,通过共聚焦激光扫描显微镜(CLSM)分析了距顶点5mm和1mm处的最大穿透深度和密封剂穿透百分比。通过扫描电子显微镜(SEM)对每组中的其余5颗牙齿进行测试涂片层评分。CLSM评估表明,该地区的增加,与NI组相比,PUI组在距根尖1和5mm处检测到封闭剂渗透的深度和百分比,EA组增加幅度更大(P<0.05)。SEM实验表明,与PUI和NI组相比,EA组的涂抹层和碎屑去除得分最低(P<0.05)。总之,在牙髓治疗期间,在根尖处的密封剂渗透方面,EA优于PUI和NI,为临床根管治疗提供了新的技术思路。
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