Abstract：
The aim of this study was the molecular imprinting polymers (MIPs) assessment as a controlled release system of ciprofloxacin. The MIPs synthesis was performed by three different methods: emulsion, bulk, and co-precipitation. Lactic acid (LA) and methacrylic acid (MA) were used as functional monomers and ethylene glycol dimethacrylate as crosslinker. Also, nonimprinted polymers (NIPs) were synthesized. MIPs and NIPs were characterized by scanning electron microscopy, Fourier Transform Infrared Reflection, specific surface area, pore size, and release kinetics. Their efficiency against Staphylococcus aureus and Escherichia coli, and their cytotoxicity in dermal fibroblast cells were proven. Results show that MIPs are mesoporous materials with a pore size between 10 and 20 nm. A higher adsorption with the co-precipitation MIP with MA as a monomer was found. The release kinetics proved that a non-Fickian process occurred and that the co-precipitation MIP with LA presented the highest release rate (90.51 mg/L) in 8 h. The minimum inhibitory concentration was found between 0.031 and 0.016 mg/L for Staphylococcus aureus and between 0.004 and 0.031 mg/L for the Escherichia coli. No cytotoxicity in cellular cultures was found; also, cellular growth was favored. This study demonstrated that MIPs present promising properties for drug administration and their application in clinical practice.
摘要：
这项研究的目的是将分子印迹聚合物（MIPs）评估为环丙沙星的控释系统。通过三种不同的方法进行MIPs合成：乳液，散装,和共沉淀。乳酸（LA）和甲基丙烯酸（MA）用作功能单体，乙二醇二甲基丙烯酸酯用作交联剂。此外，合成了非印迹聚合物(NIPs)。MIP和NIP通过扫描电子显微镜进行表征，傅里叶变换红外反射,比表面积,孔径,和释放动力学。它们对金黄色葡萄球菌和大肠杆菌的功效，并证明了它们在真皮成纤维细胞中的细胞毒性。结果表明，MIP是介孔材料，孔径在10至20nm之间。发现以MA为单体的共沉淀MIP具有更高的吸附。释放动力学证明发生了非Fickian过程，并且与LA共沉淀的MIP在8h内表现出最高的释放速率（90.51mg/L）。金黄色葡萄球菌的最低抑菌浓度为0.031至0.016mg/L，大肠杆菌的最低抑菌浓度为0.004至0.031mg/L。在细胞培养物中没有发现细胞毒性；此外，细胞生长是有利的。这项研究表明，MIP为药物施用及其在临床实践中的应用提供了有希望的特性。
