PDF(Pubmed)
Abstract：
A-galactosidases (AGALs), the oligosaccharide (RFO) catabolic genes of the raffinose family, play crucial roles in plant growth and development and in adversity stress. They can break down the non-reducing terminal galactose residues of glycolipids and sugar chains. In this study, the whole genome of AGALs was analyzed. Bioinformatics analysis was conducted to analyze members of the AGAL family in Gossypium hirsutum, Gossypium arboreum, Gossypium barbadense, and Gossypium raimondii. Meanwhile, RT-qPCR was carried out to analyze the expression patterns of AGAL family members in different tissues of terrestrial cotton. It was found that a series of environmental factors stimulated the expression of the GhAGAL3 gene. The function of GhAGAL3 was verified through virus-induced gene silencing (VIGS). As a result, GhAGAL3 gene silencing resulted in milder wilting of seedlings than the controls, and a significant increase in the raffinose content in cotton, indicating that GhAGAL3 responded to NaCl stress. The increase in raffinose content improved the tolerance of cotton. Findings in this study lay an important foundation for further research on the role of the GhAGAL3 gene family in the molecular mechanism of abiotic stress resistance in cotton.
摘要：
A-半乳糖苷酶（AGAL），棉子糖家族的寡糖（RFO）分解代谢基因，在植物生长发育和逆境胁迫中起着至关重要的作用。它们可以分解糖脂和糖链的非还原末端半乳糖残基。在这项研究中,分析了AGALs的全基因组。对陆地棉AGAL家族成员进行生物信息学分析,植物园棉,巴巴多斯棉属,还有灵蒙地羊.同时,采用RT-qPCR方法分析了AGAL家族成员在陆地棉不同组织中的表达模式。发现一系列环境因素刺激GhAGAL3基因的表达。通过病毒诱导的基因沉默（VIGS）验证了GhAGAL3的功能。因此,GhAGAL3基因沉默导致幼苗比对照更温和的枯萎，棉花中棉子糖含量显著增加，表明GhAGAL3对NaCl胁迫有反应。棉子糖含量的增加提高了棉花的耐受性。本研究的研究结果为进一步研究GhAGAL3基因家族在棉花抗逆性分子机制中的作用奠定了重要基础。
