PDF(Pubmed)
Abstract：
Francisella tularensis, causative agent of tularemia, is a contagious zoonotic ailment. This study was aimed to molecularly detect F. tularensis in tortoise blood (n = 100) and ticks (n = 100) collected in the West Azerbaijan province, Iran suing a 16SrRNA gene of the Francisella genus through employment of the Nested-PCR technique. The identified ticks were s Hyalomma aegyptium by morphological analysis. Seven percent (with a 95% CI: 3.5%-13.75%) of animal blood samples yielded positive results for the presence of the Francisella. Meanwhile, the Francisella was identified in tick samples at a rate of fifteen percent (15%) (with a 95% CI: 9%-23%). The samples containing positive results were specifically classified as F. tularensis subsp. holarctica. The samples were taken from ticks belonging to the H. aegyptium species that were gathered in Oshnavieh, southern part of West Azerbaijan province, Iran. This research was aimed to validate the existence of F. tularensis in ticks found within the West Azerbaijan province. Consequently, it is vital to acknowledge the potential of these ticks to transmit the bacteria to both livestock and humans through tick bites in this specific area.
摘要：
图拉西斯,Tularemia的病原体，是一种具有传染性的人畜共患疾病.这项研究旨在分子检测在西阿塞拜疆省收集的乌龟血液（n=100）和壁虱（n=100）中的土拉灵。伊朗通过采用Nested-PCR技术起诉Francisella属的16SrRNA基因。通过形态学分析，鉴定出的蜱为水霉。7％（95％CI：3.5％-13.75％）的动物血液样本对Francisella的存在产生了阳性结果。同时,在蜱虫样本中发现Francisella的比例为15%(15%)(95%CI:9%-23%).含有阳性结果的样品被具体地分类为土力氏杆菌亚种。holarctica.样本取自在Oshnaveyeh收集的属于埃及伊蚊的蜱虫，西阿塞拜疆省南部，伊朗。这项研究的目的是验证在西阿塞拜疆省发现的蜱中是否存在土拉伦斯。因此,重要的是要承认这些蜱通过蜱虫叮咬将细菌传播给牲畜和人类的潜力。
