PDF(Pubmed)
Abstract：
During the cryopreservation of sperm, the production of highly reactive oxygen species (ROS) can reduce their viability and fertility. However, the addition of antioxidants can help reduce the harmful effects of ROS. One such antioxidant is selenium, which is a co-factor of the glutathione peroxidase enzyme that is effective in scavenging ROS. Cysteamine can also take part in the structure of this enzyme. The use of nanoparticles can be less toxic to cells than their salt form. To this end, researchers synthesized Se-NPs using the streptococcus bacteria and loaded cysteamine onto the synthesized Se-NPs. The biosynthesis of Se-NPs and cysteamine loaded on Se-NPs was confirmed by UV-visible spectroscopy, X-ray diffraction (EDX), Fourier transforms infrared (FTIR) spectroscopy, and Field Emission Scanning Electron Microscope (FE-SEM). For cryopreservation, ram semen samples were diluted, and different concentrations (0, 1, 5, 25, and 125 µg/mL) of cysteamine, Se-NPs, cysteamine loaded on Se-NPs, and sodium selenite were added. An extender containing no supplement was considered as control group. After cooling the semen samples, they were frozen and stored in liquid nitrogen for evaluation. The samples were thawed and analyzed for mobility, viability, membrane and DNA integrity, and sperm abnormalities, as well as malondialdehyde level (MDA) and superoxide dismutase (SOD). The data was processed using SPSS, and a significance level of p < 0.05 was considered. The results of this experiment showed that adding 1 μg/mL of cysteamine loaded on Se-NPs to the diluent significantly increased the motility, viability, and membrane integrity and SOD of spermatozoa compared to the other treatment groups and control group, and reduced the abnormality, apoptosis, and MDA level of spermatozoa in comparison with the other treatment groups and control group (p < 0.05). In conclusion, the addition of cysteamine loaded on Se-NPs was found to improve the quality of ram sperm after cryopreservation.
摘要：
在冷冻保存精子的过程中,高活性氧（ROS）的产生会降低其生存能力和生育能力。然而,添加抗氧化剂可以帮助减少ROS的有害影响。一种这样的抗氧化剂是硒，它是谷胱甘肽过氧化物酶的辅助因子，可有效清除ROS。半胱胺也可以参与该酶的结构。纳米颗粒的使用可以比它们的盐形式对细胞的毒性更小。为此,研究人员使用链球菌细菌合成Se-NP，并将半胱胺加载到合成的Se-NP上。Se-NP和半胱胺负载在Se-NP的生物合成通过紫外可见光谱证实，X射线衍射(EDX),傅里叶变换红外(FTIR)光谱,场发射扫描电子显微镜（FE-SEM）。对于冷冻保存，RAM精液样本被稀释，和不同浓度（0、1、5、25和125µg/mL）的半胱胺，Se-NP,半胱胺负载在Se-NP上，并加入亚硒酸钠。不含补充剂的补充剂被视为对照组。精液样本冷却后,将它们冷冻并储存在液氮中用于评估。样品解冻并分析流动性，生存能力，膜和DNA的完整性，和精子异常，以及丙二醛水平（MDA）和超氧化物歧化酶（SOD）。数据使用SPSS处理，并且认为P<0.05的显著性水平。该实验的结果表明，向稀释剂中添加1μg/mL负载在Se-NP上的半胱胺显着增加了运动性，生存能力，与其他治疗组和对照组相比，精子的膜完整性和SOD，减少了异常，凋亡，精子MDA水平与其他治疗组和对照组比较(p<0.05)。总之,发现在Se-NPs上添加半胱胺可以改善冷冻保存后公羊精子的质量。
