RESULTS: Low and deep anaesthesia corresponded to median isoflurane concentrations of 1.3% and 1.5%, respectively, with no significant differences in heart rate (p = 0.74). Low anaesthesia resulted in shorter aortic blood clearance half-life (p = 0.091) and increased relative renal tracer influx rate (p = 0.018). A tendency toward earlier Tmax occurred under low anaesthesia (p = 0.063) with no differences in T50 (p = 0.40) and T25 (p = 0.24). Variance increased with deep anaesthesia. Compared to single mouse scans, hotel mice in position 1 showed a delayed Tmax, T50, and T25 (p < 0.05 each). Furthermore, hotel mice in position 1 showed delayed Tmax versus position 3, and delayed T50 and T25 versus position 2 and 3 (p < 0.05 each). No difference occurred between single bed and positions 2 (p = 1.0) and 3 (p = 1.0).
CONCLUSIONS: Deep anaesthesia and prolonged low anaesthesia should be avoided during renal scintigraphy because they result in prolonged blood clearance half-life, delayed renal influx and/or later Tmax. Vice versa, low anaesthesia with high respiratory rates of 80-90 rpm and short duration (≤ 20 min) should be preferred to obtain representative data with low variance.
结果:低度和深度麻醉对应的异氟醚浓度中位数为1.3%和1.5%,分别,心率无显著差异(p=0.74)。低麻醉导致较短的主动脉血液清除半衰期(p=0.091)和增加的相对肾示踪剂流入率(p=0.018)。在低麻醉状态下(p=0.063),T50(p=0.40)和T25(p=0.24)没有差异。差异随着深度麻醉而增加。与单只鼠标扫描相比,位置1的酒店老鼠表现出延迟的Tmax,T50和T25(各p<0.05)。此外,位置1的旅馆小鼠与位置3相比显示出延迟的Tmax,与位置2和3相比显示出延迟的T50和T25(各p<0.05)。在单床和位置2(p=1.0)和3(p=1.0)之间没有差异。
结论:肾闪烁扫描期间应避免深麻醉和长时间的低麻醉,因为它们会导致延长的血液清除半衰期,延迟肾脏内流和/或延迟Tmax。反之亦然,应首选低麻醉、高呼吸频率80-90rpm和短持续时间(≤20分钟),以获得低方差的代表性数据.