PDF(Pubmed)
Abstract：
BACKGROUND: The influence of anaesthetic depth and the potential influence of different anaesthetic beds and thus different handling procedures were investigated in 86 severe combined immunodeficient (SCID) mice using semi-stationary dynamic single photon emission computed tomography (SPECT) for kidney scintigraphy. Therefore, isoflurane concentrations were adjusted using respiratory rate for low (80-90 breath/min) and deep anaesthesia (40-45 breath/min). At low anaesthesia, we additionally tested the influence of single bed versus 3-mouse bed hotel; the hotel mice were anaesthetized consecutively at ~ 30, 20, and 10 min before tracer injections for positions 1, 2, and 3, respectively. Intravenous [99mTc]Tc-MAG3 injection of ~ 28 MBq was performed after SPECT start. Time-activity curves were used to calculate time-to-peak (Tmax), T50 (50% clearance) and T25 (75% clearance).
RESULTS: Low and deep anaesthesia corresponded to median isoflurane concentrations of 1.3% and 1.5%, respectively, with no significant differences in heart rate (p = 0.74). Low anaesthesia resulted in shorter aortic blood clearance half-life (p = 0.091) and increased relative renal tracer influx rate (p = 0.018). A tendency toward earlier Tmax occurred under low anaesthesia (p = 0.063) with no differences in T50 (p = 0.40) and T25 (p = 0.24). Variance increased with deep anaesthesia. Compared to single mouse scans, hotel mice in position 1 showed a delayed Tmax, T50, and T25 (p < 0.05 each). Furthermore, hotel mice in position 1 showed delayed Tmax versus position 3, and delayed T50 and T25 versus position 2 and 3 (p < 0.05 each). No difference occurred between single bed and positions 2 (p = 1.0) and 3 (p = 1.0).
CONCLUSIONS: Deep anaesthesia and prolonged low anaesthesia should be avoided during renal scintigraphy because they result in prolonged blood clearance half-life, delayed renal influx and/or later Tmax. Vice versa, low anaesthesia with high respiratory rates of 80-90 rpm and short duration (≤ 20 min) should be preferred to obtain representative data with low variance.
RESULTS: Low and deep anaesthesia corresponded to median isoflurane concentrations of 1.3% and 1.5%, respectively, with no significant differences in heart rate (p = 0.74). Low anaesthesia resulted in shorter aortic blood clearance half-life (p = 0.091) and increased relative renal tracer influx rate (p = 0.018). A tendency toward earlier Tmax occurred under low anaesthesia (p = 0.063) with no differences in T50 (p = 0.40) and T25 (p = 0.24). Variance increased with deep anaesthesia. Compared to single mouse scans, hotel mice in position 1 showed a delayed Tmax, T50, and T25 (p < 0.05 each). Furthermore, hotel mice in position 1 showed delayed Tmax versus position 3, and delayed T50 and T25 versus position 2 and 3 (p < 0.05 each). No difference occurred between single bed and positions 2 (p = 1.0) and 3 (p = 1.0).
CONCLUSIONS: Deep anaesthesia and prolonged low anaesthesia should be avoided during renal scintigraphy because they result in prolonged blood clearance half-life, delayed renal influx and/or later Tmax. Vice versa, low anaesthesia with high respiratory rates of 80-90 rpm and short duration (≤ 20 min) should be preferred to obtain representative data with low variance.
摘要：
背景：使用半平稳动态单光子发射计算机断层扫描（SPECT）对86例重度联合免疫缺陷（SCID）小鼠进行了研究，研究了麻醉深度的影响以及不同麻醉床的潜在影响以及不同的处理程序。因此,使用低呼吸频率（80-90呼吸/分钟）和深麻醉（40-45呼吸/分钟）调整异氟烷浓度。在低麻醉时，我们还测试了单人床对3只老鼠床酒店的影响；分别在位置1、2和3的示踪剂注射前〜30、20和10分钟对酒店小鼠进行了连续麻醉。在SPECT开始后进行~28MBq的[99mTc]Tc-MAG3静脉注射。时间-活动曲线用于计算峰值时间(Tmax)，T50(50%间隙)和T25(75%间隙)。
结果：低度和深度麻醉对应的异氟醚浓度中位数为1.3%和1.5%，分别,心率无显著差异(p=0.74)。低麻醉导致较短的主动脉血液清除半衰期（p=0.091）和增加的相对肾示踪剂流入率（p=0.018）。在低麻醉状态下（p=0.063），T50（p=0.40）和T25（p=0.24）没有差异。差异随着深度麻醉而增加。与单只鼠标扫描相比，位置1的酒店老鼠表现出延迟的Tmax，T50和T25(各p<0.05)。此外，位置1的旅馆小鼠与位置3相比显示出延迟的Tmax，与位置2和3相比显示出延迟的T50和T25（各p<0.05）。在单床和位置2(p=1.0)和3(p=1.0)之间没有差异。
结论：肾闪烁扫描期间应避免深麻醉和长时间的低麻醉，因为它们会导致延长的血液清除半衰期，延迟肾脏内流和/或延迟Tmax。反之亦然,应首选低麻醉、高呼吸频率80-90rpm和短持续时间(≤20分钟),以获得低方差的代表性数据.
结果：低度和深度麻醉对应的异氟醚浓度中位数为1.3%和1.5%，分别,心率无显著差异(p=0.74)。低麻醉导致较短的主动脉血液清除半衰期（p=0.091）和增加的相对肾示踪剂流入率（p=0.018）。在低麻醉状态下（p=0.063），T50（p=0.40）和T25（p=0.24）没有差异。差异随着深度麻醉而增加。与单只鼠标扫描相比，位置1的酒店老鼠表现出延迟的Tmax，T50和T25(各p<0.05)。此外，位置1的旅馆小鼠与位置3相比显示出延迟的Tmax，与位置2和3相比显示出延迟的T50和T25（各p<0.05）。在单床和位置2(p=1.0)和3(p=1.0)之间没有差异。
结论：肾闪烁扫描期间应避免深麻醉和长时间的低麻醉，因为它们会导致延长的血液清除半衰期，延迟肾脏内流和/或延迟Tmax。反之亦然,应首选低麻醉、高呼吸频率80-90rpm和短持续时间(≤20分钟),以获得低方差的代表性数据.
