Abstract：
It is generally believed that the regulation of gene expression involves protein translation occurring before RNA transcription. Therefore, it is crucial to investigate protein translation and its regulation. Recent advancements in biological sciences, particularly in the field of omics, have revolutionized protein translation research. These studies not only help characterize changes in protein translation during specific biological or pathological processes but also have significant implications in disease prevention and treatment. In this review, we summarize the latest methods in ribosome-based translation omics. We specifically focus on the application of fluorescence imaging technology and omics technology in studying overall protein translation. Additionally, we analyze the advantages, disadvantages, and application of these experimental methods, aiming to provide valuable insights and references to researchers studying translation.
摘要：
一般认为,基因表达的调节涉及在RNA转录之前发生的蛋白质翻译。因此,研究蛋白质翻译及其调控至关重要。生物科学的最新进展，特别是在经济学领域，彻底改变了蛋白质翻译研究。这些研究不仅有助于表征特定生物学或病理过程中蛋白质翻译的变化，而且在疾病预防和治疗中具有重要意义。在这次审查中,我们总结了基于核糖体的翻译组学的最新方法。我们特别关注荧光成像技术和组学技术在研究整体蛋白质翻译中的应用。此外,我们分析优势，缺点,以及这些实验方法的应用，旨在为研究翻译的研究者提供有价值的见解和参考。
